Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,525,3500GC41.7% 63.7 / 72.7 84P257A (CCA→GCA) gltXglutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/49);  new base C (0/35);  total (0/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTG  >  W3110S.gb/2525335‑2525395
               |                                             
tcatcaGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:548748/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATGACGATACTGCATTACGCTGACTGCCCCGTg  <  1:649315/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATATTGCATTACGCTGACTGCCCCGTg  <  1:195004/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:45056/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:57006/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:557095/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:528875/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:499908/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:466819/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:454208/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:583482/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:413911/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:101194/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:384500/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:380296/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:366042/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:338777/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:578910/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:410160/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:584764/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:59050/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:593130/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:6008/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:609016/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:636866/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:6406/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:675524/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:676369/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:69832/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:701368/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:72194/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:304485/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:19069/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:198139/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:213740/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:218527/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:222246/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:233233/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:234030/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:242541/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:249387/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:285362/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:149655/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:290137/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:338399/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:290285/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:12747/61‑1 (MQ=255)
tCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCACGTg  <  1:209821/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGGATTACGCTGACTGCCCCGTg  <  1:487516/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTTCGCTGACTGCCCCGTg  <  1:704163/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTAGGCTGACTGCCCCGTg  <  1:563515/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:119197/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:614950/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:634723/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:189038/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:71044/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:176878/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:641411/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:132606/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:168678/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:690447/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:649778/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:683064/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:661530/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:136054/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:273496/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:349271/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:350384/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:300953/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:37200/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:377456/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:294353/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:404430/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:407408/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:413743/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:614119/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:479248/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:505660/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:216296/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:572574/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:20959/61‑1 (MQ=255)
tCAGCAGTGCTTCTGCCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:207218/61‑1 (MQ=255)
   gcagTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:637164/58‑1 (MQ=255)
             tGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTg  <  1:191625/48‑1 (MQ=255)
               |                                             
TCAGCAGTGCTTCTGGCAAATAACCGTCATCACGATACTGCATTACGCTGACTGCCCCGTG  >  W3110S.gb/2525335‑2525395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑