| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 873,511 | G→A | E37E (GAG→GAA) | yliE → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 873,511 | 0 | G | A | 99.6% | 514.1 / NA | 256 | E37E (GAG→GAA) | yliE | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (255/0); minor base C (1/0); total (256/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 257 total aligned reads displayed.
TGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGAT > W3110S.gb/873472‑873532 | tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCTGAACGCGCGGTAAAACg > 1:33413/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGACCGCGCGGTAAAACg > 1:149187/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTaaaa > 1:193123/1‑52 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTGAAACg > 1:545120/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAAc > 1:303070/1‑53 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAAc > 1:335925/1‑53 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAAc > 1:164360/1‑53 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:519931/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:57734/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:573636/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:559151/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:549555/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:540854/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:533518/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:532549/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:523688/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:460355/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:508975/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:507240/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:502428/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:49907/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:4914/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:486899/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:479657/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:473635/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:468596/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:462436/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:108064/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:673073/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:91275/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:87796/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:85885/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:82261/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:73315/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:71579/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:708918/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:696864/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:692646/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:689293/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:67946/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:582137/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:666838/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:661307/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:645345/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:643065/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:630401/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:626800/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:623769/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:615086/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:604554/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:600176/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:589314/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:184779/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:290579/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:285869/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:27079/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:262629/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:249564/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:237059/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:221941/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:208246/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:197110/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:194905/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:189476/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:446456/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:173424/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:172397/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:163094/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:158634/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:15001/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:135877/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:129706/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:125910/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:123043/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:101049/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:307704/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:452809/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:438065/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:429338/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:409343/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:405525/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:402725/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:393366/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:391429/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:387661/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:382338/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:379096/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:369918/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:363443/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:350929/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:343989/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:327715/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:321668/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:320637/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:314009/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:308141/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACGTGCAGa > 1:421938/1‑60 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAACGCCCGGTAAAACg > 1:429838/1‑54 (MQ=255)tGGTCTTTTTTTCATCATTAACTATCAACTGGTATAGGAACGCGCGGTAAAACg > 1:94271/1‑54 (MQ=255)tGGTC‑TTTTTTCATCATTAACTATCAACTGGTATCGGAACGCGCGGTAAAACg > 1:675838/1‑53 (MQ=38) | TGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGAT > W3110S.gb/873472‑873532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |