| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 917,926 | G→T | S344S (TCG→TCT) | ybjD → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 917,926 | 0 | G | T | 99.8% | 1311.3 / ‑6.5 | 520 | S344S (TCG→TCT) | ybjD | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (519/0); minor base A (1/0); total (520/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 521 total aligned reads displayed.
GCATGTTTGCCGACTGGTACGTGAGTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGG > W3110S.gb/917896‑917958 | gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGt > 1:660809/1‑36 (MQ=37)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTg > 1:550216/1‑38 (MQ=37)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGt > 1:702291/1‑49 (MQ=39)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTc > 1:675787/1‑50 (MQ=39)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTgg > 1:151229/1‑53 (MQ=39)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTg > 1:551903/1‑52 (MQ=39)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGTGACCGAGTg > 1:630897/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGCCCGAGt > 1:682501/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGCGt > 1:581164/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAg > 1:117879/1‑60 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:104487/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:296747/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:540510/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:341431/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:518642/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:228599/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:694338/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:82702/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:145288/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:526717/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:433514/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:95719/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:436361/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:545336/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:443992/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:448425/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:536315/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:471799/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:45090/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:50989/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:500083/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:490/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:460104/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:484657/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:646653/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:100605/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:91136/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:73170/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:709903/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:705859/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:691346/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:669922/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:665002/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:651711/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:573413/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:640206/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:636024/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:613784/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:608368/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:603539/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:594144/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:585688/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:577522/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:205445/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:279859/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:271654/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:265484/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:257509/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:249006/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:24281/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:233868/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:225164/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:218972/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:212602/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:207676/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:282772/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:190784/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:184986/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:181753/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:166114/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:158745/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:140066/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:133116/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:126154/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:123384/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:112622/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:370206/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:418389/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:409127/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:402602/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:399389/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:392352/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:387675/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:385625/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:379934/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:376524/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:373903/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:42705/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:361243/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:352315/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:335003/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:330003/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:324779/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:319492/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:31273/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:309803/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:30138/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:287631/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCT‑GGACCGAGTgg > 1:507451/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCCCCTGGCGTCTGGGACCGAGTg > 1:622495/1‑62 (MQ=255) | GCATGTTTGCCGACTGGTACGTGAGTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGG > W3110S.gb/917896‑917958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |