| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 917,950 | G→A | G352G (GGG→GGA) | ybjD → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 917,950 | 0 | G | A | 99.4% | 967.2 / NA | 488 | G352G (GGG→GGA) | ybjD | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (484/0); minor base C (3/0); total (488/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 488 total aligned reads displayed.
GCATGTTTGCCGACTGGTACGTGAGTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGG > W3110S.gb/917896‑917958 | gCATGTTTGCCGTCTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:190419/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGTGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:95803/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCTTTGCCGCCTGGCGTCTGGGACCGAGt > 1:438047/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCTTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:386040/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGTGTCTGGGACCGAGTg > 1:101981/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGCCCGAGTg > 1:281797/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAg > 1:117879/1‑60 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:363388/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:228599/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:76485/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:632479/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:545434/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:180130/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:694338/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:537886/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGt > 1:676262/1‑61 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:530065/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:507897/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:541148/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:551223/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:560104/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:576420/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:579970/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:519376/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:512829/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:604686/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:500083/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:490/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:484657/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:471799/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:459335/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:452581/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:449183/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:44421/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:656777/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:100605/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:91897/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:90190/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:712186/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:707001/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:702503/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:691346/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:686111/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:669270/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:664938/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:582845/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:649683/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:643605/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:639022/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:625899/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:619062/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:609745/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:418389/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:596440/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:587079/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:210713/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:2896/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:286006/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:277409/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:268848/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:264140/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:257334/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:249006/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:24281/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:233868/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:2226/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:215347/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:300073/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:206323/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:199762/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:123384/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:184815/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:163564/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:155282/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:150968/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:145179/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:139414/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:132388/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:374473/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:433514/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:42705/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:126016/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:408745/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:402602/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:399389/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:392630/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:388729/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:381178/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:377208/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:112622/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:371524/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:358761/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:347321/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:339268/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:331870/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:328270/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:319904/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:315872/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:31270/1‑62 (MQ=255)gCATGTTTGCCGACTGGTACGAGAGTCCTCTCGCGTTGCCGCCTGGCGTCTGGGACCGAGTg > 1:309677/1‑62 (MQ=255) | GCATGTTTGCCGACTGGTACGTGAGTCCTCGCGCGTTGCCGCCTGGCGTCTGGGGCCGAGTGG > W3110S.gb/917896‑917958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |