Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,286,571 G→A G237G (GGC→GGT)  yejK ← nucleotide associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,286,5710GA99.7% 664.4 / ‑6.5 323G237G (GGC→GGT) yejKnucleotide associated protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (321/0);  minor base T (1/0);  total (323/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 323 total aligned reads displayed.

TTCGATCTCTTCGCCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGT  >  W3110S.gb/2286559‑2286620
            |                                                 
ttCAATCTCTTCTCCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:479699/1‑62 (MQ=255)
ttCAATCTCTTCCCCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:270559/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAg                        >  1:605733/1‑40 (MQ=37)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATaaa                 >  1:420727/1‑47 (MQ=38)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGgc        >  1:220256/1‑56 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACg   >  1:702757/1‑61 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACg   >  1:641152/1‑61 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:493540/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:546720/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:544794/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:533998/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:5251/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:518416/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:512409/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:509198/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:501480/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:497717/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:585888/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:490332/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:484482/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:477121/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:4760/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:472042/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:457309/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:450836/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:44756/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:439132/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:43636/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:662577/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:92562/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:76276/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:73724/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:713067/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:704388/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:700020/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:689288/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:682390/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:67743/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:669941/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:559394/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:658890/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:651538/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:649213/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:62245/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:613493/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:601730/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:597969/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:590318/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:430565/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:568131/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:197930/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:100763/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:26192/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:255624/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:253198/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:246847/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:243138/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:239523/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:227596/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:226098/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:205163/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:200334/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:275611/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:188267/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:183712/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:165057/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:154170/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:151974/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:144668/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:139404/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:123632/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:117204/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:110822/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:107677/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:360248/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:425004/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:415855/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:411377/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:404204/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:400673/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:388971/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:385393/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:372781/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:369562/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:36766/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:284486/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:356798/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:351676/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:341854/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:335556/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:331592/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:328448/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:310757/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:303149/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:299341/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:28996/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCAAGt  >  1:614738/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCAGCTGCTCAGTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:81988/1‑62 (MQ=255)
ttCAATCTCTTCACCTGCCTGCACCTGCTCATTACAGTAGCAATAAACCTGCTGGCGCACGt  >  1:555483/1‑62 (MQ=38)
ttCAATCTCTTCACCTGCCTCCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGt  >  1:363592/1‑62 (MQ=255)
            |                                                 
TTCGATCTCTTCGCCTGCCTGCAGCTGCTCATTACAGTAGCTATAAACCTGCTGGCGCACGT  >  W3110S.gb/2286559‑2286620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑