| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,420,634 | C→T | L148L (CTG→TTG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,420,634 | 0 | C | T | 99.7% | 772.4 / ‑3.5 | 377 | coding (442/2145 nt) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (0/376); minor base . (0/1); total (0/377) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
Only 100 of 377 total aligned reads displayed.
TTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGACCCGCAACA > W3110S.gb/2420602‑2420664 | tATTTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:388192/62‑1 (MQ=255) tATGTCTCTGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATAGAACTGACTCGCAACa < 1:71636/62‑1 (MQ=255) tATGTCTCAGGTCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:456597/62‑1 (MQ=255) tATGTCTCAGGGCACTGTTACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:433481/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGACCTGACTCGCAACa < 1:220460/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAATTGACTCGCAACa < 1:600543/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCATCa < 1:682474/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:484004/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:573487/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:567783/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:557408/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:55178/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:549369/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:540400/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:531968/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:506906/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:503737/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:490905/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:580128/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:47765/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:458511/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:440196/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:42455/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:102911/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:416485/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:404992/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:656050/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:9190/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:83355/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:7773/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:712972/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:707575/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:699869/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:695197/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:679481/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:664025/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:657711/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:576778/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:646299/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:637867/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:62413/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:616024/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:608039/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:595038/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:588717/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:422846/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:578633/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:189916/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:402229/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:270863/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:26325/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:253826/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:245285/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:243279/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:235261/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:224575/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:206632/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:199550/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:196779/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:194205/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:27225/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:18232/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:1677/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:160761/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:155177/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:148922/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:144835/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:142031/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:134796/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:125984/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:116419/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:108905/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:285209/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:34957/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:345394/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:339501/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:333810/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:32546/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:355521/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:362778/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:3770/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:386737/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:323097/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:316608/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:39796/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:313342/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:307090/62‑1 (MQ=255) tATGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACCCGCAACa < 1:666167/62‑1 (MQ=255) aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:446416/61‑1 (MQ=255) aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:407850/61‑1 (MQ=255) aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:514079/61‑1 (MQ=255) aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:519644/61‑1 (MQ=255) aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:525590/61‑1 (MQ=255) aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:544642/61‑1 (MQ=255) aTGTCTCAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:297656/61‑1 (MQ=255) tcAGGGCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:218497/56‑1 (MQ=255) gCACTGATACTCCGGAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:630132/51‑1 (MQ=255) ggAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:360681/38‑1 (MQ=37) ggAACAGTTGAAAGAGCGTATCGAACTGACTCGCAACa < 1:43093/38‑1 (MQ=37) aCAGTTGAAAGAGCGTATCGAACTGCCTCGCAACa < 1:371267/35‑1 (MQ=25) | TTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGACCCGCAACA > W3110S.gb/2420602‑2420664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |