Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,645,156 A→G L276L (TTG→CTG)  pbpC ← fused transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,645,1560AG100.0% 842.1 / NA 307L276L (TTG→CTG) pbpCfused transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/307);  total (0/307)

Only 100 of 307 total aligned reads displayed.

AGACGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCG  >  W3110S.gb/2645131‑2645190
                         |                                  
agtcgtcgtTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:90012/57‑1 (MQ=255)
agtcgtcgtTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:194115/57‑1 (MQ=255)
aGACGTCGTTGAGGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:152502/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGGTTTTACCg  <  1:209294/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:484956/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:582604/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:57221/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:56685/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:554396/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:548233/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:541598/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:538425/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:528711/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:518478/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:502519/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:486571/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:594516/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:481759/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:472891/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:459468/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:448892/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:442714/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:438177/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:431586/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:428217/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:109044/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:414087/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:663468/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:94972/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:83302/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:77664/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:711736/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:704275/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:700295/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:68692/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:683352/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:670898/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:665836/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:587118/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:660742/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:652731/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:645414/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:630081/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:616257/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:610662/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:605639/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:601642/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:422494/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:590118/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:185598/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:271433/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:411399/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:256784/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:248948/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:235066/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:226738/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:221992/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:214637/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:211828/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:1999/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:190352/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:260293/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:182077/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:178638/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:17704/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:170922/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:165028/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:158751/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:144309/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:137788/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:130875/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:117263/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:323408/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:409413/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:39203/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:39005/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:387536/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:371925/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:364032/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:354756/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:34662/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:338237/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:275281/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:318318/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:313683/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:306303/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:302665/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:298820/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:285822/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATCTTGTCGCTTTTGCTTTTACCg  <  1:516718/60‑1 (MQ=255)
aGACGTCGTCGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:52668/60‑1 (MQ=255)
 gACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:301475/59‑1 (MQ=255)
 gACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:127983/59‑1 (MQ=255)
       gtTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:126644/53‑1 (MQ=255)
           aaGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:239076/49‑1 (MQ=255)
             gACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:614499/47‑1 (MQ=255)
                    aTCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:522935/40‑1 (MQ=37)
                     tCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:693396/39‑1 (MQ=37)
                     tCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:383962/39‑1 (MQ=37)
                     tCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:551743/39‑1 (MQ=37)
                         |                                  
AGACGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCG  >  W3110S.gb/2645131‑2645190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑