| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,218,176 | G→A | S18S (TCG→TCA) | ygjG → | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,218,176 | 0 | G | A | 99.6% | 568.6 / ‑6.9 | 276 | S18S (TCG→TCA) | ygjG | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (275/0); total (276/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 276 total aligned reads displayed.
ATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAA > W3110S.gb/3218145‑3218205 | atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGc > 1:138033/1‑40 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTg > 1:402372/1‑43 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAgcgc > 1:585227/1‑49 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGccc > 1:324078/1‑51 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCa > 1:235207/1‑52 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGccc > 1:426602/1‑57 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGccc > 1:663373/1‑57 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGccc > 1:214099/1‑57 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGccc > 1:361792/1‑57 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGccc > 1:388549/1‑57 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:573488/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:571066/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:575857/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:583403/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:623932/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:589921/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:559798/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:551629/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:537075/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:527957/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:523255/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:512739/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:500783/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:496320/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:480689/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:472868/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:471663/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:460187/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:671231/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:86114/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:81288/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:71647/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:714409/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:710336/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:700198/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:694785/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:691181/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:685445/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:67885/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:674291/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:603630/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:669675/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:659008/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:653509/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:649453/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:647366/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:642756/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:639865/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:63607/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:628610/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:419167/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:605485/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:176832/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:440708/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:270178/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:258888/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:251498/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:242086/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:235993/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:210171/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:205147/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:199527/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:18567/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:18222/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:279983/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:167791/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:159778/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:152297/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:146957/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:145522/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:12882/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:124078/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:123259/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:11295/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:105697/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:336587/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:399086/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:380280/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:375810/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:410616/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:41756/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:365305/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:355643/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:344751/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:342767/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:328041/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:321601/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:318766/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:308486/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:298257/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:102876/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:29388/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:284393/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCTGaa > 1:446231/1‑61 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTCCAGCGCCCACGCCCt > 1:265129/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGATTTAGCGTGCAGCGCCCACGCCCt > 1:595412/1‑58 (MQ=255)atatTTTGAACAGGTTACCTTCGAGCGCATCAGATATAGCGTGCAg > 1:366702/1‑46 (MQ=38)atatTTTGAACAGGTTACCGTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCCCGccc > 1:163726/1‑57 (MQ=255)atatTTTAAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:465957/1‑58 (MQ=255)aaatTTTGAACAGGTTACCTTCGAGCGCATCAGCTTTAGCGTGCAGCGCCCACGCCCt > 1:477446/3‑58 (MQ=255) | ATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAA > W3110S.gb/3218145‑3218205 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |