Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,065,200 C→A L49L (CTG→CTT)  yhgN ← predicted antibiotic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,065,2000CA99.7% 930.2 / ‑6.0 368L49L (CTG→CTT) yhgNpredicted antibiotic transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (0/367);  minor base T (0/1);  total (0/368)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 368 total aligned reads displayed.

AGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTC  >  W3110S.gb/4065192‑4065253
        |                                                     
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:484557/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:549285/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:540191/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:533090/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:527865/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:524751/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:52352/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:51520/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:508964/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:502018/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:493883/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:490047/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:555695/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:480282/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:470025/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:465271/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:459976/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:456467/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:454704/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:44941/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:445070/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:101923/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:432686/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:411948/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:644776/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:92389/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:8714/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:85090/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:80694/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:712222/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:704371/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:683377/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:669734/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:662668/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:659112/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:653962/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:441486/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:638402/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:633542/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:631564/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:62812/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:617948/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:617134/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:615450/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:585953/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:578051/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:566526/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:561929/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:177586/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:258714/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:256427/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:403609/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:238711/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:232570/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:227644/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:213937/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:20818/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:20410/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:191674/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:185340/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:248903/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:174107/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:165496/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:163445/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:156556/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:151583/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:145257/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:131134/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:121173/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:119536/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:113440/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:111567/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:337452/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:398055/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:38849/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:38200/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:373794/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:367594/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:360031/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:356377/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:349660/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:345158/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:278422/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:333988/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:332109/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:327009/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:324596/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:323757/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:319245/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:303200/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:299636/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:29266/62‑1 (MQ=255)
aGCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:286203/62‑1 (MQ=255)
 gCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:622887/61‑1 (MQ=255)
 gCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:672289/61‑1 (MQ=255)
 gCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:312995/61‑1 (MQ=255)
 gCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:696533/61‑1 (MQ=255)
 gCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:594896/61‑1 (MQ=255)
 gCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:73899/61‑1 (MQ=255)
 gCATCACAAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTc  <  1:375195/61‑1 (MQ=255)
        |                                                     
AGCATCACCAGGAGAGCAATAAGCAACTCTCGCACCATGATTGCCCGCCGTCTTTTCGGTTC  >  W3110S.gb/4065192‑4065253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑