Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,210,809	C→A	G125V (GGT→GTT)	yrdA ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,210,809	0	C	A	100.0%	28.7 / NA	14	G125V (GGT→GTT)	yrdA	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/14);  total (0/14)

CTCTCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCACCAATCATCACATCATCTTCTA  >  W3110S.gb/4210770‑4210830                                        |                      ctctCCAGGCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:176461/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:164534/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:168085/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:178524/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:231109/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:342860/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:441432/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:486763/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:553094/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:651712/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:69028/61‑1 (MQ=255) ctctCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:707266/61‑1 (MQ=255)  tctcCAGCCGTTTATTTTGTGGGACCAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:102344/60‑1 (MQ=255)                           cAGACTACCCGCAACAATCATCACATCATCTTcta  <  1:373090/35‑1 (MQ=255)                                        |                      CTCTCCAGCCGTTTATTTTGTGGGACCAGACTACCCGCACCAATCATCACATCATCTTCTA  >  W3110S.gb/4210770‑4210830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑