Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 271,155 T→C S34S (AGT→AGC)  insO → partial transposase of insertion element IS911A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb271,1550TC100.0% 431.3 / NA 163S34S (AGT→AGC) insOpartial transposase of insertion element IS911A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/163);  total (0/163)

Only 100 of 163 total aligned reads displayed.

AAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGC  >  W3110S.gb/271110‑271171
                                             |                
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:574146/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:225957/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:239223/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:240177/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:267566/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:633999/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:272971/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:274110/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:626032/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:593211/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:591661/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:316039/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:320799/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:350749/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:266074/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:384897/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:400224/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:560351/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:54744/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:544423/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:439210/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:441436/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:464056/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:473550/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:507026/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:478134/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:501720/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:503888/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:171713/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:135121/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:690436/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:688581/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:126441/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:155769/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:163847/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:165690/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:166779/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:691855/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:666959/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:665748/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:664349/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:660672/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:112597/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:188707/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:195489/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:200040/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:7900/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:203631/62‑1 (MQ=255)
aaaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:83247/62‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACtcc  <  1:10026/61‑3 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:570552/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:78821/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:517813/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:520154/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:505384/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:532517/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:677161/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:671549/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:641685/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:577097/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:599719/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:660373/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:420785/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:640233/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:657447/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:270446/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:104905/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:110043/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:117021/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:119559/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:13821/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:14560/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:168309/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:174465/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:174713/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:178734/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:218865/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:259236/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:489889/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:291123/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:425033/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:474067/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:466673/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:441715/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:292847/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:42326/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:406945/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:354235/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:345736/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:314256/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:304507/61‑1 (MQ=255)
 aaCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGAATTACGCAGCCAGGTACTTGAACTGc  <  1:465017/61‑1 (MQ=255)
  aCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:222850/60‑1 (MQ=255)
       ccTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:369730/55‑1 (MQ=255)
       ccTGAAAAGCCAGACGGCAGACGGGCTGTATTACGAAGCCAGGTACTTGAACTGc  <  1:151462/55‑1 (MQ=17)
        cTGCAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:186055/54‑1 (MQ=17)
        cTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:537612/54‑1 (MQ=255)
            aaaGCCAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:540448/50‑1 (MQ=255)
                ccAGACGGCAGACGGGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:152911/46‑1 (MQ=255)
                      ggcagacggGCTGTATTACGCAGCCAGGTACTTGAACTGc  <  1:711073/40‑1 (MQ=255)
                                             |                
AAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGC  >  W3110S.gb/271110‑271171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑