Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 486,896 T→C V379V (GTT→GTC)  kefA → fused mechanosensitive channel proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb486,8960TC100.0% 684.0 / NA 256V379V (GTT→GTC) kefAfused mechanosensitive channel proteins
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/256);  total (0/256)

Only 100 of 256 total aligned reads displayed.

GACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGC  >  W3110S.gb/486854‑486903
                                          |       
gACGAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:603796/50‑1 (MQ=255)
gACCAACCGCATTGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:687689/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:469488/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:557635/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:547834/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:543101/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:532170/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:521883/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:515013/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:507760/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:49859/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:495591/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:493140/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:490857/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:564204/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:457162/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:447871/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:446259/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:444138/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:441685/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:431728/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:422408/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:416659/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:103070/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:406011/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:631326/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:89624/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:714719/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:711175/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:701133/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:697610/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:673280/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:669011/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:659491/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:64502/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:643452/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:632948/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:56150/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:624901/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:622725/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:619171/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:60026/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:596220/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:589652/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:581447/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:575335/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:572853/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:409787/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:161376/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:226809/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:397638/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:212312/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:209062/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:19992/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:190699/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:182187/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:179812/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:16804/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:167118/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:16325/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:213001/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:157190/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:15303/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:146733/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:141183/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:135678/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:132854/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:12707/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:125150/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:120251/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:110912/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:106972/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:315471/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:391213/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:37950/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:375735/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:367784/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:360906/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:348189/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:335645/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:334026/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:320876/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:233047/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:313339/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:306548/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:303140/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:297/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:286380/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:27781/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:26149/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:259584/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:254107/50‑1 (MQ=255)
gACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:250561/50‑1 (MQ=255)
 aCCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:476484/49‑1 (MQ=255)
 aCCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:171321/49‑1 (MQ=255)
 aCCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:324082/49‑1 (MQ=255)
 aCCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:691479/49‑1 (MQ=255)
  ccAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:65337/48‑1 (MQ=255)
         cATCGCGGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:216256/41‑1 (MQ=255)
             gcgGATTTGCGTCTCGAACAGTTTGAAGTCAACcagc  <  1:652262/37‑1 (MQ=255)
                                          |       
GACCAACCGCATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGC  >  W3110S.gb/486854‑486903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑