Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,645,164 2 bp→AC coding (817‑818/2313 nt) pbpC ← fused transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,645,1640GA100.0% 773.8 / NA 308T273V (ACT→GTT) pbpCfused transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/308);  total (0/308)
*W3110S.gb2,645,1650TC100.0% 820.4 / NA 308T273V (ACT→GTT) pbpCfused transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/308);  total (0/308)

Only 100 of 308 total aligned reads displayed.

AGACGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCG  >  W3110S.gb/2645131‑2645190
                                 ||                         
agtcgtcgtTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:90012/57‑1 (MQ=255)
agtcgtcgtTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:194115/57‑1 (MQ=255)
aGATGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:32279/60‑1 (MQ=255)
aGACGTCGTTGAGGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:152502/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGGTTTTACCg  <  1:209294/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:485977/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:570030/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:560306/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:554396/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:548233/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:541598/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:538425/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:528711/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:518478/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:516116/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:499858/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:594516/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:483427/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:480198/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:472891/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:459468/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:448892/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:442714/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:438177/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:431586/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:428217/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:109044/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:662903/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:94972/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:83302/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:77664/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:711736/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:704275/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:700295/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:68692/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:683352/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:670898/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:665836/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:587118/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:656273/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:652731/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:645414/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:630081/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:616257/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:610662/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:605639/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:601642/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:422494/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:590118/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:187332/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:271433/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:260293/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:256784/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:248948/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:23103/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:223590/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:220789/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:213950/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:211828/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:1999/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:190352/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:411202/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:184463/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:178638/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:17704/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:170922/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:165028/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:158751/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:144309/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:137788/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:130875/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:117263/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:338237/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:413877/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:40744/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:391101/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:389404/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:378777/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:371925/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:364032/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:354756/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:34662/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:275011/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:317491/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:309444/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:304357/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:302430/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:301233/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:29139/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:2858/60‑1 (MQ=255)
aGACGTCGTTGAAGACCGGCACCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:386569/60‑1 (MQ=255)
aGACGTCGTCGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:52668/60‑1 (MQ=255)
 gACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:236547/59‑1 (MQ=255)
 gACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:57860/59‑1 (MQ=255)
 gACGTCGTTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:127983/59‑1 (MQ=255)
       gtTGAAGACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:126644/53‑1 (MQ=255)
             gACCGGCATCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:614499/47‑1 (MQ=255)
                    aTCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:522935/40‑1 (MQ=37)
                     tCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:693396/39‑1 (MQ=37)
                     tCCAGCGTAGTAACGATTTTGTCGCTTTTGCTTTTACCg  <  1:551743/39‑1 (MQ=37)
                                 ||                         
AGACGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCG  >  W3110S.gb/2645131‑2645190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑