Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 135,820 G→A D215D (GAC→GAT)  speE ← spermidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb135,8200GA100.0% 957.4 / NA 441D215D (GAC→GAT) speEspermidine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (441/0);  total (441/0)

Only 100 of 441 total aligned reads displayed.

TCGGGATCGCCGCCTGATAAAAGCCAACGTCGCTGAAGTAATGGCTG  >  W3110S.gb/135792‑135838
                            |                  
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCt   >  1:105014/1‑46 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCt   >  1:212492/1‑46 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:327376/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:253134/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:32400/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:322778/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:31978/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:315495/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:308800/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:304588/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:302068/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:298055/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:293008/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:283506/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:279862/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:277552/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:275445/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:273524/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:270381/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:267383/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:264743/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:263085/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:261341/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:258240/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:256227/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:9554/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:5552/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:101255/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:92002/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:87964/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:84102/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:79560/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:75136/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:73282/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:67732/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:64684/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:62186/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:59457/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:57389/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:330518/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:54493/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:52469/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:48404/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:46288/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:43440/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:41202/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:39069/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:35538/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:344874/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:339955/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:333242/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:14198/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:250947/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:177273/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:174590/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:171279/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:168953/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:166733/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:160266/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:157100/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:151682/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:149392/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:145018/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:178843/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:136278/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:133703/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:129261/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:128056/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:127095/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:122431/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:119037/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:115773/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:111048/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:108294/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:107399/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:182518/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:24925/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:246446/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:24312/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:242159/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:239457/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:238423/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:234647/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:233097/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:228297/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:225615/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:219865/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:215962/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:208888/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:201474/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:19726/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:193994/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:192573/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:189532/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:185169/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:179851/1‑47 (MQ=255)
tCGGGATCGCCGCCTGATAAAAGCCAACATCGCTGAACTAATGGCTg  >  1:168285/1‑47 (MQ=38)
tCGGGATCGCCGCCTGATAAAAGCCAACAACGCTGAAGTAATGCCt   >  1:162534/1‑46 (MQ=38)
tCGGGATCGCCGCCTGAAAAAAGCCAACATCGCTGAAGTAATGGCTg  >  1:223140/1‑47 (MQ=38)
tCGCGATCGCCGCCTGATAAAAGCCAACATCGCTGAAGTAATg      >  1:287876/1‑43 (MQ=38)
                            |                  
TCGGGATCGCCGCCTGATAAAAGCCAACGTCGCTGAAGTAATGGCTG  >  W3110S.gb/135792‑135838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑