| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 567,798 | C→T | A87A (GCC→GCT) | emrE → | multidrug resistance protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 567,798 | 0 | C | T | 99.8% | 1070.5 / NA | 559 | A87A (GCC→GCT) | emrE | multidrug resistance protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (558/0); minor base A (1/0); total (559/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 559 total aligned reads displayed.
TGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGC > W3110S.gb/567746‑567807 | tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgtc > 1:169669/1‑60 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:153349/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:203037/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:198942/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:323770/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:320837/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:228869/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:4074/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:163466/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:160450/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:151730/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:241490/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:78763/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:95009/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:109331/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:89833/1‑61 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:307677/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:298595/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:293980/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:259300/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:300998/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:31619/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:315176/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:313397/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:309238/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:289695/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:278286/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:295772/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:280117/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:283265/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:285906/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:305737/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:302108/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:276478/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:51667/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:92179/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:83561/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:75877/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:71882/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:68561/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:6673/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:65313/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:62393/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:56528/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:54086/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:329152/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:47762/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:44978/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:37866/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:34742/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:34509/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:342680/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:341063/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:337790/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:334398/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:140308/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:187090/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:179990/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:177965/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:174695/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:165739/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:158998/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:155826/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:148345/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:146081/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:143380/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:268495/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:137793/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:134337/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:132/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:12767/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:125226/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:121631/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:117604/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:115916/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:113546/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:103211/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:233327/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:26964/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:266564/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:265760/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:263683/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:100801/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:24986/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:245735/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:244105/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:237958/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:236211/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:225214/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:222708/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:220944/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:218311/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:215984/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:212717/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:208884/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:195770/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:192164/1‑62 (MQ=255)tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGATATCATAGGc > 1:254214/1‑62 (MQ=255)tGATTAGTATGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:183541/1‑62 (MQ=255)tGAATAGTTTGCTTTCCTGGGGATATTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:189206/1‑61 (MQ=255) | TGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGC > W3110S.gb/567746‑567807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |