| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 332,479 | C→T | D295D (GAC→GAT) | yahA → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 332,479 | 0 | C | T | 99.8% | 970.5 / NA | 514 | D295D (GAC→GAT) | yahA | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (513/0); minor base A (1/0); total (514/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 514 total aligned reads displayed.
CAAATGGCGAGTGTCGACGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTAGCGC > W3110S.gb/332462‑332522 | cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgctc > 1:18770/1‑59 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:292706/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:325976/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:322185/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:31828/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:315549/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:313713/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:310245/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:307403/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:305521/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:301605/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:297364/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:295757/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:335741/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:288626/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:282245/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:280579/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:277263/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:275022/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:271889/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:268352/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:265738/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:263513/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:261659/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:257808/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:61873/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:974/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:95880/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:94579/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:91185/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:87794/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:85507/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:80994/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:7757/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:74959/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:71063/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:67657/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:65540/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:254609/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:59714/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:56910/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:54169/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:51135/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:46352/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:4493/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:40931/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:37249/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:346549/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:343832/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:338405/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:143214/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:177308/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:17482/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:172189/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:170510/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:166556/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:161609/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:159862/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:157591/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:154702/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:151469/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:150182/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:147154/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:252515/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:139407/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:134238/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:131211/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:126332/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:118591/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:116867/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:115575/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:113775/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:112443/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:10680/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:102977/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:217104/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:100206/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:239975/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:237633/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:236306/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:233992/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:230743/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:228990/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:225072/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:221253/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:219084/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:207870/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:20538/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:201072/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:198783/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:196309/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:193729/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:192109/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:185146/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:180857/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcg > 1:212422/1‑60 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcg > 1:330756/1‑60 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTACGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:248899/1‑61 (MQ=255)cAAATGGCGAGTGTCGAAGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:246121/1‑61 (MQ=255)cAAATGGCGAGTCTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:179296/1‑61 (MQ=255) | CAAATGGCGAGTGTCGACGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTAGCGC > W3110S.gb/332462‑332522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |