| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,830,700 | G→C | A82A (GCC→GCG) | norR ← | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,830,700 | 0 | G | C | 99.8% | 896.9 / NA | 499 | A82A (GCC→GCG) | norR | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (0/498); minor base T (0/1); total (0/499) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
Only 100 of 499 total aligned reads displayed.
GTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTC > W3110S.gb/2830673‑2830735 | tGCTGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:204356/60‑1 (MQ=255) tGCGGGAAAGCGCTCAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:155156/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:281669/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:320524/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:319301/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:312943/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:309213/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:306809/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:300255/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:297682/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:293994/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:290348/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:28644/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:282732/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:321855/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:280163/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:273305/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:268924/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:268341/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:263378/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:258155/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:25424/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:251317/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:247498/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:244888/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:100836/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:53272/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:95426/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:93717/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:9051/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:8462/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:78845/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:73563/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:7155/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:68289/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:65923/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:62862/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:58152/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:242583/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:49564/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:47311/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:42702/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:3866/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:37210/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:345003/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:338425/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:336775/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:333132/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:330448/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:324509/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:126165/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:167818/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:152983/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:14943/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:145826/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:143829/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:141470/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:136287/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:134522/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:132471/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:129224/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:127350/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:236229/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:124505/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:118524/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:116975/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:114561/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:110756/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:109716/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:107131/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:104453/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:10399/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:102896/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:203665/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:239667/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:235093/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:232407/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:229890/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:225721/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:222587/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:216630/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:212533/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:209413/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:206194/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:169918/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:2026/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:198607/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:191941/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:189248/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:186833/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:183160/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:179603/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:177021/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:174212/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGACGCCCTTc < 1:87603/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCGTCCAGCCGTGGATGCCCTTc < 1:162979/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCACTTCCAGCCGGGGATGCCCTTc < 1:27722/60‑1 (MQ=255) tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAAGCGCTTCCAGCCGTGGATGCCCTTc < 1:12087/60‑1 (MQ=255) tGCGGGAAACCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc < 1:315258/60‑1 (MQ=255) gCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATCCCCTTc < 1:158489/59‑1 (MQ=255) | GTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTC > W3110S.gb/2830673‑2830735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |