| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,498,895 | T→G | intergenic (+146/‑129) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,498,895 | 0 | T | G | 99.8% | 916.7 / NA | 515 | intergenic (+146/‑129) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (514/0); total (515/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 515 total aligned reads displayed.
GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA > W3110S.gb/3498872‑3498933 | ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAACCTTCCa > 1:118892/1‑49 (MQ=21)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTTCACCTTGTTCgggtg > 1:145582/1‑60 (MQ=21)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCCCCTTGTTCgggtg > 1:317539/1‑60 (MQ=21)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgtt > 1:167532/1‑56 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgt > 1:152743/1‑55 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACctt > 1:2809/1‑53 (MQ=21)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:333/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:340517/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:330291/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:328280/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:326982/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:323711/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:321509/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:3129/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:307696/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:299501/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:294804/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:29251/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:288117/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:28554/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:283281/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:277214/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:275456/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:273286/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:270263/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:26645/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:261447/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:55241/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:96782/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:92326/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:86496/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:84200/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:81149/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:75872/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:72233/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:66387/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:6519/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:60374/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:58432/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:336851/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:53279/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:50760/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:48911/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:44793/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:41622/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:37566/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:348644/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:343365/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:109097/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:338132/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:148456/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:197111/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:195624/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:190552/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:186898/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:183777/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:182729/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:172191/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:162584/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:161086/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:157471/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:149922/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:193931/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:139931/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:137012/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:134784/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:132664/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:130969/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:127566/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:121364/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:115019/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:113346/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:100527/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:227091/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:253722/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:252454/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:248886/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:246474/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:245273/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:242837/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:241160/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:238438/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:234124/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:232389/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:199744/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:225666/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:220625/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:215597/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:21311/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:211275/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:208217/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:2051/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt > 1:179345/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt > 1:125864/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt > 1:95189/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTACCACCTTGTTCgggtg > 1:302772/1‑60 (MQ=25)ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTCAAACTTCCACcttgtt > 1:310322/1‑56 (MQ=21)ggCGTTCACGCCGCATCCGGCACGCTGTGCAAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:25895/1‑60 (MQ=21)ggCGTTCACGCCGCATCCGGCAAGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:263320/1‑60 (MQ=18)ggCGTTCACGCCGCATACGGCACGCTGTGCCAGTAATCTGAAACtt > 1:174928/1‑45 (MQ=25) | GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA > W3110S.gb/3498872‑3498933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |