Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,995,489 C→G A85A (GCC→GCG)  prlC → oligopeptidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,995,4890CG100.0% 347.8 / NA 195A85A (GCC→GCG) prlColigopeptidase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (195/0);  total (195/0)

Only 100 of 195 total aligned reads displayed.

GAACTGCGTGAAGCCTACGAACAAACCCTGCCGCTGCTGTCGGAA  >  W3110S.gb/3995475‑3995519
              |                              
gAACTGCGTGAAGCGTACGAACACACCCTGCCGCTGCTGTCAGaa  >  1:72517/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGctgctg        >  1:203352/1‑39 (MQ=255)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:279022/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:326156/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:323301/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:321911/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:318489/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:29849/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:295568/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:291293/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:288904/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:282555/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:279960/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:279646/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:334526/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:277538/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:268954/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:264472/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:26357/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:255872/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:25482/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:251620/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:24826/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:101238/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:239633/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:54750/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:93919/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:86544/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:85122/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:83685/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:80712/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:7743/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:75814/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:73230/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:70236/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:67819/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:59552/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:330096/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:51875/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:4725/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:45605/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:40340/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:38881/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:37714/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:37253/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:36331/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:34537/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:337597/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:243870/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:139852/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:239165/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:173415/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:171650/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:169055/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:166932/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:158984/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:157741/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:155812/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:152289/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:149740/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:141845/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:179019/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:137586/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:133236/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:130535/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:129340/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:123017/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:119876/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:119343/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:116927/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:115166/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:109391/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:104108/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:212893/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:236210/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:234657/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:23203/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:231783/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:227757/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:225070/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:220606/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:218532/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:216165/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:214527/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:183439/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:211808/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:211384/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:208858/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:200970/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:200198/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:194130/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:19155/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:188049/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:185764/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGa   >  1:53744/1‑44 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGa   >  1:61223/1‑44 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGa   >  1:32777/1‑44 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTCCTGTCAGaa  >  1:122104/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACACTGCCGCTGCTGTCAGaa  >  1:224398/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACACTGCCGCTGCTGTCAGaa  >  1:304812/1‑45 (MQ=38)
              |                              
GAACTGCGTGAAGCCTACGAACAAACCCTGCCGCTGCTGTCGGAA  >  W3110S.gb/3995475‑3995519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑