Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,995,516 G→A S94S (TCG→TCA)  prlC → oligopeptidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,995,5160GA100.0% 415.4 / NA 193S94S (TCG→TCA) prlColigopeptidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (193/0);  total (193/0)

Only 100 of 193 total aligned reads displayed.

GAACTGCGTGAAGCCTACGAACAAACCCTGCCGCTGCTGTCGGAA  >  W3110S.gb/3995475‑3995519
                                         |   
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:279603/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:328621/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:326791/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:326156/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:323301/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:321911/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:318489/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:29849/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:295568/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:291293/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:288904/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:282555/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:279960/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:332777/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:278420/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:269771/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:268954/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:264472/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:26357/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:255872/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:25482/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:251620/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:24826/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:101238/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:239633/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:60338/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:93919/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:86544/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:83780/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:81764/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:79985/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:7653/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:74428/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:73039/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:71600/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:68238/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:67576/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:243870/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:59425/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:54538/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:51875/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:4725/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:45605/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:40340/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:3853/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:37663/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:3700/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:35971/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:34081/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:139852/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:239165/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:173415/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:171650/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:169055/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:166646/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:157955/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:157288/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:152627/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:152264/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:144898/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:141845/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:179019/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:137586/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:133236/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:130535/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:129340/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:123017/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:119876/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:119343/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:116927/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:115166/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:109391/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:104108/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:211808/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:236210/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:234657/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:23203/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:231783/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:227757/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:226311/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:224431/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:220606/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:218532/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:216165/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:214527/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:212893/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:183439/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:211384/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:208858/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:202754/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:200376/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:198722/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:193117/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:19155/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:188049/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGaa  >  1:185764/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTGCTGTCAGa   >  1:53744/1‑44 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACCCTGCCGCTCCTGTCAGaa  >  1:122104/1‑45 (MQ=38)
gAACTGCGTGAAGCGTACGAACAAACACTGCCGCTGCTGTCAGaa  >  1:304812/1‑45 (MQ=38)
gAACTGCGTGAAGCGTAAGAACAAACCCTGCCGCTGCTGACAGaa  >  1:336748/1‑45 (MQ=25)
                                         |   
GAACTGCGTGAAGCCTACGAACAAACCCTGCCGCTGCTGTCGGAA  >  W3110S.gb/3995475‑3995519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑