Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,041,397 A→G K213K (AAA→AAG)  livJ → leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,041,3970AG100.0% 1106.6 / NA 527K213K (AAA→AAG) livJleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/527);  total (0/527)

Only 100 of 528 total aligned reads displayed.

GGCGCGTCTGAAAAAAGAGAATATCGACTTCGTTTACTACGGCGGTTATCACCCGGAAATGG  >  W3110S.gb/4041385‑4041446
            |                                                 
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACTGCGGTTACCACCCGGAAATgg  <  1:72215/62‑1 (MQ=17)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCTGAAATgg  <  1:52876/62‑1 (MQ=17)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:290712/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:329177/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:324741/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:324127/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:320846/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:317803/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:313806/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:312482/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:305131/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:30003/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:296542/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:294255/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:339177/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:288182/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:285707/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:282623/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:279905/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:272755/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:269640/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:264033/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:257553/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:254603/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:100707/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:248649/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:65279/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:9694/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:95014/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:92575/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:87659/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:86525/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:85041/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:81775/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:78385/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:76362/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:73853/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:69374/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:333844/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:63070/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:58476/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:55008/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:51532/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:44767/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:4258/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:39050/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:347935/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:340793/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:251510/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:138854/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:175070/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:169050/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:167661/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:165287/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:161889/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:157384/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:156001/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:152392/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:148560/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:147320/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:141580/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:139721/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:177839/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:135661/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:131626/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:129247/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:126117/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:1222/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:116527/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:112869/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:111789/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:108670/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:104995/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:1032/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:207025/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:235138/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:231337/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:228130/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:239732/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:224228/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:221977/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:218178/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:216275/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:212595/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:246270/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:210194/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:204474/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:202452/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:24088/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:19593/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:194221/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:191631/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:190042/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:182526/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:180288/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:245578/62‑1 (MQ=255)
 gcgcGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:67500/61‑1 (MQ=255)
 gcgcGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:276851/61‑1 (MQ=255)
 gcgcGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:197334/61‑1 (MQ=255)
 gcgcGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:331980/61‑1 (MQ=255)
            |                                                 
GGCGCGTCTGAAAAAAGAGAATATCGACTTCGTTTACTACGGCGGTTATCACCCGGAAATGG  >  W3110S.gb/4041385‑4041446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑