Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 33,903 | T→C | I1029I (ATT→ATC) | carB → | carbamoyl‑phosphate synthase large subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 33,903 | 0 | T | C | 99.6% | 562.7 / ‑6.3 | 243 | I1029I (ATT→ATC) | carB | carbamoyl‑phosphate synthase large subunit |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (0/242); minor base A (0/1); total (0/243) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 |
Only 100 of 243 total aligned reads displayed.
TTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACC > W3110S.gb/33884‑33945 | ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:68818/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:753928/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:749835/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:738937/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:732016/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:729776/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:721674/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:713073/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:707504/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:704923/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:70135/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:694601/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:761324/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:677722/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:655592/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:649548/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:615647/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:586046/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:573938/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:553173/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:532236/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:519305/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:1003856/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:495236/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:872050/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:969784/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:965549/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:958760/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:955130/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:943193/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:924099/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:918143/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:916423/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:896452/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:89359/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:889515/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:508274/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:857397/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:844920/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:834336/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:824820/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:821687/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:808980/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:798725/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:77842/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:776165/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:768778/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:168056/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:309341/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:277572/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:273652/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:271257/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:235606/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:230869/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:225971/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:222780/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:212543/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:204873/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:173506/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:303407/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:156587/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:147934/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:139253/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:132447/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:124779/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:115978/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:110169/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:1025289/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:1013524/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:1005302/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:1005041/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:332478/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:475250/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:473879/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:454901/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:44644/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:440639/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:432155/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:430351/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:424771/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:419032/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:409393/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:397109/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:352005/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:321367/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:336708/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:343129/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:359837/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:369254/62‑1 (MQ=255) ttGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:376115/62‑1 (MQ=255) tGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCATTATAAAGTGCATTACGACaccacc < 1:776347/61‑1 (MQ=255) tGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:385527/61‑1 (MQ=255) tGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:545653/61‑1 (MQ=255) tGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:840365/61‑1 (MQ=255) tGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:288860/61‑1 (MQ=255) tGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:515875/61‑1 (MQ=255) tGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:487377/61‑1 (MQ=255) tGAAGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:504978/61‑1 (MQ=255) aaGACTCCCGCGTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:669374/59‑1 (MQ=255) cgTGATCCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACaccacc < 1:189017/49‑1 (MQ=255) | TTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCACC > W3110S.gb/33884‑33945 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |