Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,965,568 T→C G399G (GGA→GGG)  flhA ← predicted flagellar export pore protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,965,5680TC99.8% 1001.9 / NA 430G399G (GGA→GGG) flhApredicted flagellar export pore protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (0/429);  minor base G (0/1);  total (0/430)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 430 total aligned reads displayed.

ATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTC  >  W3110S.gb/1965529‑1965589
                                       |                     
aTCTATATTGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:706020/61‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:582427/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:716051/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:699230/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:6781/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:670155/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:654673/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:640306/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:615386/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:611856/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:60395/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:597317/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:72005/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:576973/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:569096/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:552719/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:540352/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:531434/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:515466/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:512470/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:498765/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:1000343/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:476569/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:4720/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:856378/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:994757/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:97649/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:966461/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:957664/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:948463/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:92210/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:894543/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:880982/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:874954/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:868471/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:863499/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:489591/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:850678/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:840263/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:809741/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:800177/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:796119/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:781836/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:763787/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:756792/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:744833/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:737015/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:722450/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:158958/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:261885/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:467157/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:242278/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:232912/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:226473/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:210890/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:207103/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:186354/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:180245/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:17558/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:16132/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:254067/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:150398/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:143515/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:138576/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:131215/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:127261/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:1043066/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:1035312/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:1028870/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:1016607/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:1007515/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:1004487/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:366444/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:459817/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:452450/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:439245/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:434553/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:425589/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:418243/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:405495/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:39406/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:386698/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:381898/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:373115/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:274067/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:347953/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:338961/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:332927/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:330559/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:309399/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:304258/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:295484/54‑1 (MQ=255)
       ttGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAACAAATTTc  <  1:912630/54‑1 (MQ=255)
       ttGTCGCGAATGTACACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:666310/54‑1 (MQ=255)
       ttGTCGCGAATCTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:814175/54‑1 (MQ=255)
       ctGTCGCGAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:354721/53‑1 (MQ=255)
              gAATGTGCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:635279/47‑1 (MQ=39)
                  gtgCACCACTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:285877/43‑1 (MQ=38)
                     caccacTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:194318/40‑1 (MQ=38)
                        cacTGGCGGCAGAAACCCCATCTCCTGAGCAAATTTc  <  1:986073/37‑1 (MQ=38)
                                       |                     
ATCCATATTGTCGCGAATGTGCACCACTGGCGGCAGAAATCCCATCTCCTGGGCAAATTTC  >  W3110S.gb/1965529‑1965589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑