Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,989,623 C→T intergenic (+58/+21) yecI → / ← yecJ predicted ferritin‑like protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,989,6230CT99.7% 880.7 / NA 362intergenic (+58/+21)yecI/yecJpredicted ferritin‑like protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (361/0);  total (362/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 362 total aligned reads displayed.

AATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTT  >  W3110S.gb/1989580‑1989641
                                           |                  
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTg              >  1:656015/1‑50 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTg              >  1:722856/1‑50 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGa             >  1:179248/1‑51 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGGAGCAGCGGttt  >  1:321471/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:69725/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:803246/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:79547/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:788071/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:778492/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:769724/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:755824/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:750735/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:746159/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:738893/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:732250/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:70492/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:822381/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:685523/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:682635/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:670178/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:643359/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:617477/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:615545/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:605395/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:59548/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:590600/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:581248/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:892941/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:988179/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:979111/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:975927/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:96608/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:964088/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:948526/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:944097/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:93079/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:925486/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:919812/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:897184/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:8118/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:883949/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:880116/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:876880/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:869381/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:861562/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:853226/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:851277/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:843763/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:831847/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:828599/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:573974/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:186623/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1002147/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:357806/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:34587/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:337652/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:311036/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:292539/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:28259/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:26157/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:244508/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:23787/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:195962/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:360132/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:17441/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:169794/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:161042/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:148946/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:137556/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:117102/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:108425/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1041706/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1033811/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1027625/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:1018317/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:437641/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:568555/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:55693/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:548606/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:543499/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:525315/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:511633/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:507303/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:498820/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:488507/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:470781/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:462355/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:450084/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:368453/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:436177/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:426096/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:417739/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:41286/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:40816/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:395867/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGttt  >  1:380730/1‑62 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGtt   >  1:227937/1‑61 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGtt   >  1:375973/1‑61 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGt    >  1:776477/1‑60 (MQ=255)
aaTGCATTGCGCCGATGAAGTTTTTGAGAAACCGCTGCCTCATTTTTTTGAAGCAGCGGatt  >  1:267069/1‑62 (MQ=255)
                                           |                  
AATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTT  >  W3110S.gb/1989580‑1989641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑