Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,164,441 C→A S63S (TCG→TCT)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,164,4410CA100.0% 652.6 / NA 266S63S (TCG→TCT) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/266);  total (0/266)

Only 100 of 266 total aligned reads displayed.

GGCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATG  >  W3110S.gb/3164438‑3164498
   |                                                         
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCGCAGTTCAGACATCGCATACACAATg  <  1:355959/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:716865/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:808426/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:804674/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:795249/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:783628/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:777766/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:767679/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:760219/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:7517/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:743198/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:734090/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:725855/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:814255/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:698790/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:696312/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:69093/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:680962/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:666115/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:647796/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:643825/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:637472/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:629738/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:612253/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:60969/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:595922/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:881103/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:987508/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:980017/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:973278/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:962798/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:957873/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:953655/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:944264/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:935760/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:923146/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:912585/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:896980/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:889849/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:590002/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:875135/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:866340/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:852636/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:848809/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:846681/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:845768/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:842183/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:841907/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:82968/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:823847/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:82179/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:185743/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:321252/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:301451/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:292135/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:275217/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:27188/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:262583/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:250564/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:24797/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:234833/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:223555/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:218081/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:200694/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:328318/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:178502/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:174922/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:161170/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:157260/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:150026/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:144130/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:130300/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:1042575/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:1022993/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:1013798/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:1008510/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:473995/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:581629/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:566626/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:543019/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:539440/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:528089/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:521750/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:520187/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:496300/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:4901/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:1000328/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:461718/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:441477/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:4320/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:427218/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:416522/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:409885/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:403363/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:394046/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:377105/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:375366/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:343197/61‑1 (MQ=255)
ggCAGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTGCAGACATCGCATACACAATg  <  1:57483/61‑1 (MQ=255)
ggCAGATGTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATg  <  1:54468/61‑1 (MQ=255)
   |                                                         
GGCCGATTTTTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATG  >  W3110S.gb/3164438‑3164498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑