| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,513,980 | T→C | T213T (ACT→ACC) | ftsN → | essential cell division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,513,980 | 0 | T | C | 99.8% | 1280.5 / NA | 559 | T213T (ACT→ACC) | ftsN | essential cell division protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (557/0); minor base A (1/0); total (559/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 559 total aligned reads displayed.
GCAGCCGTACCAGGATCTGCTGCAAACTCCTGCGCACACGACTGCGCAGTCGAAGCCGCAGCA > W3110S.gb/3513953‑3514015 | gcagcCGTACCAGGATCTGCTGCAAACCCCTGCTCACACGACCGCGCAGTCGAAGCCgca > 1:826906/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGcac > 1:485281/1‑37 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGca > 1:754864/1‑36 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACg > 1:205241/1‑40 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGAc > 1:334614/1‑42 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:662869/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:747177/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:717839/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:540594/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:708891/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:698136/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:694879/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:690961/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:681474/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:67497/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:559981/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:583961/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:658510/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:64953/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:570064/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:644470/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:634384/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:626849/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:620099/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:609386/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:59262/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:58746/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:864901/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:990998/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:977136/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:967284/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:957650/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:949378/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:941440/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:935764/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:917127/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:906574/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:90146/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:879015/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:728734/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:853756/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:846474/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:834376/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:815108/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:807648/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:796785/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:790265/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:779557/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:773335/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:512923/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:741670/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:196039/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:30241/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:291551/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:281625/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:274472/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:267628/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:261675/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:256032/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:249693/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:243746/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:233344/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:219579/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:213010/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:31584/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:18894/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:167244/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:15070/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:130252/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:126848/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:121470/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:108151/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:104644/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:1037615/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:1018396/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:1008472/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:427704/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:1000945/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:503071/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:495527/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:476676/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:472040/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:465418/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:460090/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:45322/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:445468/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:434601/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:533123/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:418010/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:411053/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:400864/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:387580/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:382943/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:380927/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:361547/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:351651/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:348622/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:343461/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca > 1:329016/1‑60 (MQ=255)gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgc > 1:600930/1‑59 (MQ=255) | GCAGCCGTACCAGGATCTGCTGCAAACTCCTGCGCACACGACTGCGCAGTCGAAGCCGCAGCA > W3110S.gb/3513953‑3514015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |