Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,513,995 T→C T218T (ACT→ACC)  ftsN → essential cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,513,9950TC100.0% 1336.5 / NA 551T218T (ACT→ACC) ftsNessential cell division protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (551/0);  total (551/0)

Only 100 of 551 total aligned reads displayed.

GCAGCCGTACCAGGATCTGCTGCAAACTCCTGCGCACACGACTGCGCAGTCGAAGCCGCAGCA  >  W3110S.gb/3513953‑3514015
                                          |                    
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACATGACCGCGCAGTCGAAGCCgca     >  1:348646/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGCCCGCGCAGTCGAAGCCgca     >  1:485225/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCa                 >  1:941335/1‑48 (MQ=38)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:626849/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:71528/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:7083/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:698136/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:693966/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:689066/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:680478/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:671866/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:662086/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:64946/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:639517/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:632779/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:727428/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:618303/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:609386/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:59929/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:59262/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:586010/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:583961/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:570064/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:559981/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:540594/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:533123/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:845964/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:990998/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:977136/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:966914/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:955448/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:935764/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:914591/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:903114/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:89847/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:878453/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:863951/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:852398/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:512923/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:833165/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:820608/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:815108/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:805586/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:796785/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:788451/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:779557/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:767872/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:754368/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:745714/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:739916/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:196039/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:30241/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:1000945/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:281625/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:267628/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:256032/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:251009/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:243746/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:233924/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:220045/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:216437/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:206471/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:291551/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:18894/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:168740/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:15070/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:133800/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:126848/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:122995/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:108151/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:104644/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:1037615/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:1018396/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:1008472/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:411053/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:503071/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:495527/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:476133/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:471300/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:465418/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:459063/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:45322/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:442937/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:434601/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:418010/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:31584/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:400864/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:387580/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:382943/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:380927/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:370021/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:351651/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:343461/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:329016/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgc      >  1:262560/1‑59 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgc      >  1:948981/1‑59 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGACgca     >  1:33477/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAACCgca     >  1:426502/1‑60 (MQ=255)
gcagcCGTACCAGGATCTGCTGCAAAC‑CCTGCGCACACGACCGCGCAGTCGAAGCCgcagca  >  1:658438/1‑62 (MQ=255)
gcagcCGTACAAGGATCTGCTGCAAACCCCTGCGCACACGACCGCGCAGTCGAAGCCgca     >  1:275606/1‑60 (MQ=255)
                                          |                    
GCAGCCGTACCAGGATCTGCTGCAAACTCCTGCGCACACGACTGCGCAGTCGAAGCCGCAGCA  >  W3110S.gb/3513953‑3514015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑