Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,132,833 G→A K39K (AAG→AAA)  yhfU → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,132,8330GA100.0% 1260.7 / NA 514K39K (AAG→AAA) yhfUhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/514);  total (0/514)

Only 100 of 514 total aligned reads displayed.

GGCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAGGTGAAATCCGG  >  W3110S.gb/4132786‑4132844
                                               |           
ggCTGTTTTGAAGTTTTTATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:880780/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCTTTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:188984/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:75604/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:793172/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:73290/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:721485/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:709650/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:702010/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:693849/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:686833/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:679282/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:659141/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:635974/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:627299/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:613056/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:604477/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:599149/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:596051/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:593305/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:577793/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:560756/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:55741/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:548844/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:534368/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:530127/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:1000261/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:896447/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:989906/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:982482/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:976958/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:964951/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:956320/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:938930/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:932369/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:92195/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:912761/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:908530/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:902677/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:775235/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:886475/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:874806/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:863303/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:852386/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:846715/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:840576/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:828716/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:826176/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:809460/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:805717/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:522414/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:226117/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:334207/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:517290/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:312839/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:304594/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:293331/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:287186/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:273768/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:265575/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:255658/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:245942/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:233011/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:324882/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:21830/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:181324/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:161300/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:146782/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:140349/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:134759/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:110265/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:1037425/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:1028809/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:1017724/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:1007614/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:339456/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:504871/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:501279/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:484765/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:476287/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:463179/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:447986/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:434897/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:428335/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:419733/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:413330/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:410728/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:401679/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:391763/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:387801/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:380397/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:369845/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:364294/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:355522/59‑1 (MQ=255)
ggCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:352437/59‑1 (MQ=255)
 gCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:584825/58‑1 (MQ=255)
 gCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:119822/58‑1 (MQ=255)
 gCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:1032963/58‑1 (MQ=255)
 gCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:495203/58‑1 (MQ=255)
 gCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGAAATGAAAGTGAAATCCgg  <  1:747858/58‑1 (MQ=255)
                       caACGACATGGAAGCGGCAATGAAAGTGAAATCCgg  <  1:204182/36‑1 (MQ=255)
                                               |           
GGCTGTTTTGAAGTTTTCATTCACAACGACATGGAAGCGGCAATGAAGGTGAAATCCGG  >  W3110S.gb/4132786‑4132844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑