Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,415,294 A→G E20E (GAA→GAG)  yjfJ → predicted transcriptional regulator effector protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,415,2940AG99.7% 858.3 / NA 370E20E (GAA→GAG) yjfJpredicted transcriptional regulator effector protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (369/0);  total (370/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 370 total aligned reads displayed.

AAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAATCCATTGAAGAAACCC  >  W3110S.gb/4415249‑4415310
                                             |                
aaGTTTATTTACGCTGGTGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTgaaga       >  1:638838/1‑57 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGTAAGAGTCCATTGAAGAAAccc  >  1:728467/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTgaagaa      >  1:951762/1‑58 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTgaagaa      >  1:675915/1‑58 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTgaag        >  1:984165/1‑56 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTgaag        >  1:519007/1‑56 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:747227/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:549351/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:735482/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:725205/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:714176/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:706988/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:684872/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:683462/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:668633/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:665273/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:645920/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:632156/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:622909/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:612803/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:604067/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:598441/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:58613/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:571828/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:568149/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:553877/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:538138/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:852113/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:971173/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:964440/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:960010/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:942026/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:937003/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:930935/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:9216/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:900351/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:892537/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:87229/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:861953/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:760837/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:841925/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:834951/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:832553/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:816111/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:810525/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:806139/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:803076/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:791263/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:783542/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:778118/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:768130/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:177880/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:1003676/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:271262/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:262969/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:244797/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:226780/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:213577/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:207244/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:204719/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:197836/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:186811/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:181386/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:295036/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:168612/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:161926/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:153550/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:144722/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:129663/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:10451/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:103819/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:1029454/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:1022182/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:1015780/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:421620/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:529165/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:50010/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:498022/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:485165/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:473663/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:45991/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:447213/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:439313/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:437243/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:434763/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:428320/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:306299/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:408430/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:403152/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:392824/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:380339/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:367471/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:358111/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:350662/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:34247/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:334820/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:314950/1‑62 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAcc   >  1:111947/1‑61 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAGTCCATTGAAGAAAc    >  1:251492/1‑60 (MQ=255)
aaGTTTATTTACGCTGGGGAAATCGTTTATCCCCCAGGCGGAAGAGTCCATTGAAGAAAccc  >  1:235981/1‑62 (MQ=255)
                                             |                
AAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAATCCATTGAAGAAACCC  >  W3110S.gb/4415249‑4415310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑