Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 4,460,351 | T→C | intergenic (‑60/‑116) | fbp ← / → mpl | fructose‑1,6‑bisphosphatase I/UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 4,460,351 | 0 | T | C | 99.8% | 1313.9 / NA | 565 | intergenic (‑60/‑116) | fbp/mpl | fructose‑1,6‑bisphosphatase I/UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (0/564); minor base G (0/1); total (0/565) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 565 total aligned reads displayed.
AATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGC > W3110S.gb/4460344‑4460405 | aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATga < 1:413458/62‑2 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:646106/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:737217/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:732813/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:725436/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:715213/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:69988/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:694524/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:688466/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:682592/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:672029/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:665016/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:65812/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:751642/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:633289/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:628120/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:622700/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:617033/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:613632/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:603343/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:595314/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:586668/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:57794/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:572660/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:566564/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:8893/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:988605/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:976736/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:965225/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:954742/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:941760/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:929564/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:919377/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:91332/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:911135/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:907257/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:900755/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:897678/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:558496/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:882438/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:870203/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:857870/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:848011/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:828834/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:824197/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:817653/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:804642/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:797330/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:788542/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:776951/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:164228/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:296963/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:280184/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:268337/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:25975/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:240047/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:228802/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:218819/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:21395/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:202843/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:188194/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:183333/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:171779/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:545879/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:159922/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:154018/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:146685/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:134661/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:120719/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:106112/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:1040181/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:1032189/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:102319/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:1009249/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:1003099/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:435637/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:1000484/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:538441/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:530049/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:518342/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:504189/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:495870/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:486727/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:480496/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:469120/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:460696/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:452661/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:439322/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:42329/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:402591/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:394754/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:379315/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:372491/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:353051/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:343612/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:327788/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:315571/62‑1 (MQ=255) aataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:308920/62‑1 (MQ=255) aataatCCAAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:76876/62‑1 (MQ=255) ataatCCCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGc < 1:3599/61‑1 (MQ=255) | AATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGATTATCCTGAAATGC > W3110S.gb/4460344‑4460405 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |