Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,473,417	T→A	C371S (TGC→AGC)	mgtA →	magnesium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,473,417	0	T	A	100.0%	33.7 / NA	17	C371S (TGC→AGC)	mgtA	magnesium transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/0);  total (17/0)

AAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCAC  >  W3110S.gb/4473360‑4473421                                                          |     aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCa   >  1:33047/1‑61 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:621133/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:992579/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:925596/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:834130/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:788137/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:783520/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:73401/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:681456/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:674362/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:1010625/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:484188/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:226230/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:176535/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:15/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:14293/1‑62 (MQ=255) aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGAGCAc  >  1:111693/1‑62 (MQ=255)                                                          |     AAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCAC  >  W3110S.gb/4473360‑4473421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑