Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,152,075 2 bp→CA coding (1047‑1048/1947 nt) yegI ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,152,0750TC100.0% 1382.7 / NA 554N350D (AAT→GAT) yegIconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (554/0);  total (554/0)
*W3110S.gb2,152,0760CA99.8% 1306.9 / NA 554G349G (GGG→GGT) yegIconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (553/0);  minor base T (1/0);  total (554/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 554 total aligned reads displayed.

AGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATA  >  W3110S.gb/2152054‑2152112
                     ||                                    
aGACTCTCGCCAGCACAAAATCACCGCCGGTGTTAATGCCCTCTTCGCCGAGATc      >  1:854963/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGAc                     >  1:387530/1‑40 (MQ=37)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTc               >  1:349327/1‑46 (MQ=37)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCg           >  1:706024/1‑50 (MQ=38)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGAt       >  1:474140/1‑54 (MQ=38)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:732294/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:753965/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:723491/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:712356/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:681259/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:671532/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:6642/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:657624/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:647401/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:642536/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:630152/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:614601/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:60695/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:60495/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:582612/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:573859/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:568493/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:553923/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:543901/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:53852/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:532259/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:845879/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:983325/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:967915/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:954570/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:94887/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:944207/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:928705/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:914717/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:901332/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:893211/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:883096/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:876483/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:864500/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:741748/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:843480/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:832558/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:82563/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:811283/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:80785/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:792502/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:786967/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:778955/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:772256/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:76471/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:1005598/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:157676/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:264799/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:255583/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:245818/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:232603/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:226848/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:212158/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:201100/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:19670/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:180340/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:173483/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:166281/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:277097/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:150350/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:141619/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:132203/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:116121/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:110937/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:1036273/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:1028480/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:1022399/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:1012738/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:1000724/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:410288/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:514/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:50869/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:499853/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:484752/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:480530/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:467094/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:45625/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:436182/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:431609/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:424408/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:292233/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:405546/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:394448/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:376755/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:365689/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:353418/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:343853/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:338521/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:32011/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCCGAGATc      >  1:303204/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGCAGAGATc      >  1:935949/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGACGAGATc      >  1:693268/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTTCGACGAGATc      >  1:527167/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGACCTCTACGCCGAGATc      >  1:220189/1‑55 (MQ=255)
aGACTCTCGCCAGCACAAAATCACCGCCGGTGGTAATGAACTCTTCGCCGAGATc      >  1:441495/1‑55 (MQ=255)
                     ||                                    
AGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATA  >  W3110S.gb/2152054‑2152112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑