Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	762,948	G→C	T335T (ACG→ACC)	sucB →	dihydrolipoyltranssuccinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	762,948	0	G	C	100.0%	15.6 / NA	11	T335T (ACG→ACC)	sucB	dihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/0);  total (11/0)

TGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCG  >  W3110S.gb/762890‑762951                                                           |    tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTAcccc   >  1:27679/1‑61 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:15843/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:16166/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:2496/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:2717/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:38362/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:45907/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:6329/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:6672/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:7867/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:7946/1‑62 (MQ=255) tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:9413/1‑62 (MQ=255)                                                           |    TGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCG  >  W3110S.gb/762890‑762951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑