Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	914,839	A→G	V147V (GTT→GTC)	ybjE ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	914,839	0	A	G	100.0%	86.5 / NA	46	V147V (GTT→GTC)	ybjE	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/46);  total (0/46)

ATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCG  >  W3110S.gb/914834‑914887      |                                                 aTACCGACGAGGAAAAGTAGCAGAATTTAGGTGTATTCACTGGCTTCGGTcgcg  <  1:5868/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAATGTGTATTCACTGGCTTCGGTcgcg  <  1:19076/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:44634/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:33672/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:33733/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:34830/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:34930/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:35132/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:36610/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:38251/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:39584/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:40497/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:42404/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:44369/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:32285/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:45290/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:45546/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:54230/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:5552/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:590/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:7680/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:8171/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:971/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:23875/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:10283/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:10550/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:12226/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:15838/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:15851/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:19809/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:2033/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:20363/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:20974/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:1005/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:24317/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:24411/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:25261/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:26388/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:26649/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:27620/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:2894/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:29170/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:293/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:31909/54‑1 (MQ=255) aTACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTAACTGGCTTCGGTcgcg  <  1:5352/54‑1 (MQ=255)  tACCGACGAGGAAAAGTAGCAGAATTAAGGTGTATTCACTGGCTTCGGTcgcg  <  1:14415/53‑1 (MQ=255)      |                                                 ATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCG  >  W3110S.gb/914834‑914887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑