Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,223,854	T→A	intergenic (+55/+28)	ycgH → / ← ymgD	ECK1157:JW5901+JW5176:b4491; hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,223,854	0	T	A	100.0%	52.2 / NA	30	intergenic (+55/+28)	ycgH/ymgD	ECK1157:JW5901+JW5176:b4491; hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (30/0);  total (30/0)

TGGGCCTGAAATATAAGTTCTGATCCCGCCGTTAGCTAAAAAACCGCGTCGTATTCATC  >  W3110S.gb/1223803‑1223861                                                    |        tggGCCTGAAATATAAGTTTTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:32433/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:28332/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:8120/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:6968/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:49960/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:46025/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:43765/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:43363/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:38616/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:37870/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:34529/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:32806/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:30031/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:29583/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:29037/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:10329/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:27786/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:27642/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:2655/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:25132/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:24027/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:23255/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:21569/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:18023/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:14588/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:13960/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:1347/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:12702/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:11477/1‑59 (MQ=255) tggGCCTGAAATATAAGTTCTGATCCCGCCATTAGCTAAAAAACCGCGTCGAATTCATc  >  1:10843/1‑59 (MQ=255)                                                    |        TGGGCCTGAAATATAAGTTCTGATCCCGCCGTTAGCTAAAAAACCGCGTCGTATTCATC  >  W3110S.gb/1223803‑1223861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑