Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,356,838	T→C	G784G (GGT→GGC)	gltB →	glutamate synthase, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,356,838	0	T	C	100.0%	115.2 / NA	58	G784G (GGT→GGC)	gltB	glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/58);  total (0/58)

CGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTG  >  W3110S.gb/3356830‑3356890         |                                                     cGCATTGGCTGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:5548/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:50491/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:37931/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:38102/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:39175/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:40125/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:40313/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:41860/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:42406/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:42603/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:46513/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:46910/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:47046/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:49359/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:49775/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:50146/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:37181/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:50557/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:50616/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:50806/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:50835/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:52047/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:5216/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:52760/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:53048/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:53569/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:7057/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:8804/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:9616/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:36273/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:12737/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:13164/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:14283/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:14804/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:15807/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:18675/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:18729/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:19664/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:20848/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:21243/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:21494/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:22481/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:22797/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:25044/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:25113/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:28177/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:2971/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:29751/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:31259/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:31818/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:3235/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:3316/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:33234/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:3397/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:34750/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:3583/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:12471/61‑1 (MQ=255) cGCATTGGCGGGGCGAGCTTTGAAGACTACCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:23764/61‑1 (MQ=255)      tggcggGGCGAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTg  <  1:6899/56‑1 (MQ=38)         |                                                     CGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTG  >  W3110S.gb/3356830‑3356890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑