| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 762,925 | 3 bp→ACT | coding (982‑984/1218 nt) | sucB → | dihydrolipoyltranssuccinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 762,925 | 0 | G | A | 100.0% | 17.0 / NA | 12 | V328T (GTG→ACT) | sucB | dihydrolipoyltranssuccinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/0); total (12/0) | |||||||||||
| * | W3110S.gb | 762,926 | 0 | T | C | 100.0% | 18.0 / NA | 12 | V328T (GTG→ACT) | sucB | dihydrolipoyltranssuccinase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/0); total (12/0) | |||||||||||
| * | W3110S.gb | 762,927 | 0 | G | T | 100.0% | 18.5 / NA | 12 | V328T (GTG→ACT) | sucB | dihydrolipoyltranssuccinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (12/0); total (12/0) | |||||||||||
TGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCG > W3110S.gb/762890‑762951 ||| tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTAcccc > 1:27679/1‑61 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:15843/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:16166/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:2496/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:2717/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:38362/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:45907/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:6329/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:6672/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:7867/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:7946/1‑62 (MQ=255)tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg > 1:9413/1‑62 (MQ=255) ||| TGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCG > W3110S.gb/762890‑762951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |