Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 762,936 2 bp→TA coding (993‑994/1218 nt) sucB → dihydrolipoyltranssuccinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb762,9360CT100.0% 18.5 / NA 12S331S (TCC→TCTsucBdihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/0);  total (12/0)
*W3110S.gb762,9370CA100.0% 17.0 / NA 12L332M (CTG→ATG) sucBdihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/0);  total (12/0)

TGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCG  >  W3110S.gb/762890‑762951
                                              ||              
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTAcccc   >  1:27679/1‑61 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:15843/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:16166/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:2496/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:2717/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:38362/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:45907/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:6329/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:6672/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:7867/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:7946/1‑62 (MQ=255)
tGACCGGCGGTACATTCAGTATTACCAACGGCGGTACTTTCGGTTCTATGATGTCTACCCCg  >  1:9413/1‑62 (MQ=255)
                                              ||              
TGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCG  >  W3110S.gb/762890‑762951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: