Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262332 3262332 1–1199 68 [0] [0] 66 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)41 (1.160) 23/92 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261064‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401
                                                                                                                                           
TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      >  1:1209663/1‑71
TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      >  1:1464034/1‑71
    CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACT                                                                     <  1:3668463/68‑1
     CTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   <  1:3035014/69‑1
      TTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   >  1:2807396/1‑68
      TTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                                         <  1:2711358/62‑1
        CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   >  1:823719/1‑66
        CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:1223750/1‑62
        CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:2365186/1‑62
        CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:2037918/1‑62
         ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              >  1:3069216/1‑70
            AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                                          <  1:885833/71‑1
            AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                           <  1:2733327/70‑1
            AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                           <  1:1390677/70‑1
             AAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAA                                                             <  1:2150022/67‑1
              AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                           <  1:573609/68‑1
              AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:479165/65‑1
                ATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATG                                                      <  1:3323160/71‑1
                ATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATG                                                      <  1:3277611/71‑1
                 TAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGG                                                     <  1:1133677/71‑1
                 TAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGG                                                     <  1:2025357/71‑1
                   ATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCA                                                   <  1:492998/71‑1
                      TCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      >  1:3155011/1‑49
                      TCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      >  1:3576153/1‑49
                            CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                           >  1:447069/1‑70
                               TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATC                                       <  1:677724/71‑1
                                 CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:2272967/1‑71
                                   TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTT                                   >  1:3337119/1‑71
                                   TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:3133966/44‑1
                                    CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  >  1:2145521/1‑71
                                    CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAA                                              >  1:2414417/1‑59
                                    CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAA                                              >  1:783813/1‑59
                                    CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:2690079/1‑45
                                     AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:2294140/70‑1
                                     AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:3569533/70‑1
                                     AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:2095643/70‑1
                                     AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:3056092/70‑1
                                      ACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTT                                 >  1:3223905/1‑70
                                      ACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAA                                                             <  1:402929/42‑1
                                       CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               >  1:1136015/1‑71
                                       CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               >  1:1732960/1‑71
                                         AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        >  1:1272779/1‑44
                                          AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTC                            <  1:1778446/71‑1
                                          AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTC                            <  1:2938790/71‑1
                                          AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTT                             <  1:783548/70‑1
                                           ATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATT                                    <  1:2473161/62‑1
                                            TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATG                                                      <  1:3590922/43‑1
                                            TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATG                                                      <  1:2569924/43‑1
                                                 CAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                           <  1:131567/49‑1
                                                     TTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGT                      <  1:120940/66‑1
                                                     TTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGT                      <  1:798124/66‑1
                                                      TCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGT                      <  1:3471744/65‑1
                                                       CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:313926/70‑1
                                                              CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCAC        <  1:2842200/71‑1
                                                                 GTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAA      <  1:861806/70‑1
                                                                 GTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCG                    <  1:1710367/56‑1
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:3351097/1‑70
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAA                 >  1:499768/1‑57
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:107780/1‑71
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    <  1:527195/69‑1
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAA              >  1:1458931/1‑59
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAA              >  1:1887193/1‑59
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTT                                >  1:2292179/1‑41
                                                                     ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:2266710/70‑1
                                                                     ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    <  1:165490/68‑1
                                                                     ACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:419078/1‑35
                                                                     ACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:2921551/1‑35
                                                                     ACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:1639428/1‑35
                                                                     ACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:1757769/1‑35
                                                                                                                                           
TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261064‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.