Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,303,633	G→T	100%	Q246Y (CAG→TAT)	oppA →	oligopeptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,303,633	0	G	T	100.0%	87.1 / NA	30	Q246Y (CAG→TAT)	oppA	oligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/11);  total (19/11)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAA  >  W3110S.gb/1303568‑1303694                                                                  |                                                              aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                          >  1:2938168/1‑71 (MQ=255) aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                          <  1:2274969/71‑1 (MQ=255)  acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                         >  1:3411918/1‑71 (MQ=255)  acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                         >  1:1704775/1‑71 (MQ=255)    gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                       <  1:505475/71‑1 (MQ=255)     aacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                      >  1:1925363/1‑71 (MQ=255)              ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                    <  1:638626/64‑1 (MQ=255)              ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                    <  1:2010169/64‑1 (MQ=255)                cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                      >  1:1425776/1‑60 (MQ=255)                cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                           >  1:230450/1‑71 (MQ=255)                cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                           >  1:2604586/1‑71 (MQ=255)                 ttGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                          >  1:3329690/1‑71 (MQ=255)                          gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt                                 <  1:3073356/71‑1 (MQ=255)                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                              >  1:1283199/1‑71 (MQ=255)                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                              >  1:2539/1‑71 (MQ=255)                               aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                       <  1:1657910/44‑1 (MQ=38)                                ccTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                           >  1:3626950/1‑71 (MQ=255)                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                     >  1:1146437/1‑41 (MQ=38)                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                     >  1:2175067/1‑41 (MQ=38)                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                       <  1:2596945/71‑1 (MQ=255)                                      ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa                      >  1:2371899/1‑70 (MQ=255)                                      ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa                      >  1:2226384/1‑70 (MQ=255)                                                 aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                    <  1:1609523/45‑1 (MQ=38)                                                   aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa                      >  1:11623/1‑57 (MQ=255)                                                    aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                     <  1:3025455/41‑1 (MQ=38)                                                       gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt            <  1:1605401/63‑1 (MQ=255)                                                        ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGa    >  1:991030/1‑70 (MQ=255)                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                            >  1:425104/1‑44 (MQ=38)                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGc                  >  1:1959993/1‑54 (MQ=255)                                                           ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaaa  <  1:55319/69‑1 (MQ=255)                                                                  |                                                              AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAA  >  W3110S.gb/1303568‑1303694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑