| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,740 | T→G | 55.9% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,740 | 0 | T | G | 55.9% | 45.2 / 40.6 | 43 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (8/11); new base G (12/12); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC > W3110S.gb/70675‑70806 | tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctt > 1:1998952/1‑71 (MQ=255)tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt > 1:871582/1‑71 (MQ=255)tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:2662053/70‑1 (MQ=255) ttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGc > 1:67313/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:3504403/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:1712175/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc > 1:3469650/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc > 1:1805807/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGAATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:236835/1‑71 (MQ=255) gcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGaa < 1:1602879/71‑1 (MQ=255) cTACTGGCATGAATGCCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg > 1:2076951/1‑70 (MQ=255) tACTGGCATGAATGGCTTAACTGGTCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:2716939/1‑71 (MQ=255) tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttc < 1:606368/51‑1 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct < 1:3607118/46‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct < 1:710140/44‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcgc < 1:2268019/62‑1 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttc > 1:1411918/1‑41 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:355574/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAAAACGGGtttct > 1:3407049/1‑42 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgtt > 1:2105037/1‑39 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt > 1:2140035/1‑71 (MQ=255) ggCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:544845/39‑1 (MQ=255) ggCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGc < 1:1543576/66‑1 (MQ=255) ggCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGc < 1:842365/66‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcgc < 1:50679/56‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:1765105/1‑69 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg < 1:2452514/70‑1 (MQ=255) aCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGa < 1:1175328/70‑1 (MQ=255) ggCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:2949903/1‑61 (MQ=255) cGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:469829/71‑1 (MQ=255) tCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg < 1:68016/37‑1 (MQ=255) tCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGa < 1:3550573/38‑1 (MQ=255) tCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg > 1:2969239/1‑71 (MQ=255) cAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg > 1:1706057/1‑70 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa > 1:2939695/1‑70 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa > 1:244302/1‑52 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAg > 1:1101021/1‑53 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:1315542/71‑1 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa < 1:2431105/69‑1 (MQ=255) tGCCAATACGGGTTTCTTTCGCCCGGATGAAgcgc < 1:36521/35‑1 (MQ=255) ccAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:531735/71‑1 (MQ=255) ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:2244043/71‑1 (MQ=255) ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:662533/71‑1 (MQ=255) ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCAGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:1151327/71‑1 (MQ=255) ccAATACGGGGGTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:865764/71‑1 (MQ=255) aaTACGGGGTTCTTTGGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg > 1:2890992/1‑54 (MQ=39) aaTACGAGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCt > 1:471734/1‑53 (MQ=39) tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:3052640/42‑1 (MQ=255) tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa < 1:1095026/60‑1 (MQ=255) cGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc < 1:2816780/71‑1 (MQ=255) cGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc < 1:2483809/70‑1 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc > 1:257468/1‑71 (MQ=255) gTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc < 1:3139144/67‑1 (MQ=255) tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCa < 1:2514177/38‑1 (MQ=255) | TACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC > W3110S.gb/70675‑70806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |