| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,514 | T→C | 100% | pseudogene (647/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,514 | 0 | T | C | 100.0% | 133.3 / NA | 41 | pseudogene (647/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/19); total (22/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGG‑GAATCGAATCCATTGTAGCGACGCAGAA > W3110S.gb/3824447‑3824564 | gACGGGCGAATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCtt > 1:113323/1‑71 (MQ=255) gggCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttttt > 1:1740989/1‑71 (MQ=255) cgcATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGc < 1:502912/71‑1 (MQ=255) tcatTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg > 1:726896/1‑71 (MQ=255) catTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCA‑CCATGAAGAGCTACTCACCttg > 1:1827341/1‑60 (MQ=255) tGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc > 1:3253443/1‑71 (MQ=255) aaGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc > 1:425592/1‑69 (MQ=255) aGTTCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:2574685/71‑1 (MQ=255) aGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:3077997/71‑1 (MQ=255) aGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:3073790/71‑1 (MQ=255) gTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑gg > 1:3015130/1‑71 (MQ=255) gACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑GGGTgaatc > 1:906219/1‑70 (MQ=255) cAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:3678080/54‑1 (MQ=255) cAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:1584609/55‑1 (MQ=255) cAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑gaatcgaatc > 1:2589909/1‑70 (MQ=255) cAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGACCGAGGGG‑gaatcgaatc < 1:1088597/70‑1 (MQ=255) aaGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCa > 1:1135389/1‑69 (MQ=255) ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTg > 1:2955416/1‑70 (MQ=255) gCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑ggg‑gga < 1:603375/56‑3 (MQ=255) cGAGCCGTTCACCCATGAAGAGCTACTCACCttgtt > 1:2663854/1‑36 (MQ=255) cGAGCCGTTCACCCATGAAGAGCTACTCACCttgtt > 1:1173645/1‑36 (MQ=255) cGAGCCGTTCACCCATGAAGAGCTACTCACCttgtt > 1:369863/1‑36 (MQ=255) cGAGCCGTTCACCCATGAAGAGCTACTCACCttgtt > 1:1546481/1‑36 (MQ=255) ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:2443089/43‑1 (MQ=38) ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:3303550/43‑1 (MQ=38) ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGAc < 1:3128187/71‑1 (MQ=255) gTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTg < 1:3146124/62‑1 (MQ=255) gTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGa > 1:2925693/1‑68 (MQ=255) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:2356098/40‑1 (MQ=38) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:1486067/40‑1 (MQ=38) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:2917101/40‑1 (MQ=38) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑GGG‑GAATCGAATCCAt > 1:1964553/1‑56 (MQ=255) ccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑gaatcg < 1:2815257/47‑1 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:3672158/35‑1 (MQ=37) gAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑gaatcgaat > 1:704675/1‑46 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGaa > 1:1095182/1‑64 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGaa > 1:1070846/1‑64 (MQ=255) agagCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGa > 1:1263837/1‑56 (MQ=255) cTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGc < 1:131486/47‑1 (MQ=255) cTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCa < 1:2816232/53‑1 (MQ=255) tCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGa > 1:168848/1‑54 (MQ=255) | GACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGG‑GAATCGAATCCATTGTAGCGACGCAGAA > W3110S.gb/3824447‑3824564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |