| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 155,463 | A→T | 100% | *247K (TAA→AAA) | ecpD ← | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 155,463 | 0 | A | T | 100.0% | 234.5 / NA | 79 | *247K (TAA→AAA) | ecpD | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (33/46); total (33/46) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA > W3110S.gb/155395‑155530 | tgatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:3795324/71‑1 (MQ=255)tgatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:1749199/71‑1 (MQ=255) gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGATTTATATCCTTGCCTGATTTCAg > 1:529519/1‑71 (MQ=255) tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc < 1:1405532/71‑1 (MQ=255) atAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt < 1:4994271/70‑1 (MQ=255) tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg < 1:2118229/71‑1 (MQ=255) aaTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg > 1:1860094/1‑70 (MQ=255) aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg > 1:1120936/1‑68 (MQ=255) aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGc < 1:3878336/70‑1 (MQ=255) aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGc < 1:1512880/70‑1 (MQ=255) ttttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa > 1:886809/1‑61 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:4235123/70‑1 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:2651461/70‑1 (MQ=255) tttAGTATATTATATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt > 1:1273824/1‑70 (MQ=255) aGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCt > 1:2555737/1‑71 (MQ=255) aGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCt > 1:1439550/1‑71 (MQ=255) gTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:3565966/56‑1 (MQ=255) gTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCt < 1:391336/70‑1 (MQ=255) tataTTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc < 1:2449578/71‑1 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt > 1:4866837/1‑62 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt > 1:300028/1‑71 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAt < 1:2441448/70‑1 (MQ=255) ataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc > 1:1899197/1‑71 (MQ=255) gTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccac > 1:3923181/1‑71 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca > 1:5093934/1‑70 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca < 1:3457743/70‑1 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca > 1:3932178/1‑70 (MQ=255) gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt > 1:3451324/1‑48 (MQ=255) gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg > 1:2544487/1‑71 (MQ=255) cTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt < 1:2092860/71‑1 (MQ=255) tATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc < 1:4547126/40‑1 (MQ=255) tATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca < 1:1451731/66‑1 (MQ=255) tATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt > 1:1379764/1‑70 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:3888676/36‑1 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:1664576/36‑1 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:366973/35‑1 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa < 1:501794/70‑1 (MQ=255) gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt > 1:3246726/1‑38 (MQ=255) gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt > 1:404227/1‑70 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:2582180/70‑1 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt > 1:1719722/1‑70 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:5100117/70‑1 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt > 1:3853735/1‑69 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTa < 1:1486098/71‑1 (MQ=255) ttCCTGCGGTATTATATCCTTGCCTGATTTCAGCCt < 1:3971630/36‑1 (MQ=37) ttCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa > 1:914611/1‑71 (MQ=255) tCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAAt > 1:3705699/1‑71 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTat < 1:3883925/69‑2 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa < 1:3340115/69‑1 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa < 1:3334363/69‑1 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc < 1:566715/60‑1 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc < 1:3284154/60‑1 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCg > 1:373851/1‑70 (MQ=255) gAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTa > 1:3629296/1‑70 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:2906494/43‑1 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:3415031/43‑1 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCGTTAATGGCGTaaa < 1:2609502/71‑1 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATTGCGTaaaa < 1:1214276/71‑1 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:2250392/71‑1 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:2445593/71‑1 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:3194288/1‑39 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:3174595/1‑39 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc < 1:2005621/43‑1 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAt > 1:2524913/1‑38 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAt > 1:871753/1‑38 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc > 1:2155611/1‑43 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg > 1:4819312/1‑70 (MQ=255) tCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacc < 1:1883289/44‑1 (MQ=255) tCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:932968/65‑1 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtt > 1:4741448/1‑71 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACt < 1:1276265/70‑1 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACttt < 1:4944172/69‑1 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg < 1:2469896/70‑1 (MQ=255) cTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGc < 1:2961288/62‑1 (MQ=255) cTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtttt > 1:939998/1‑68 (MQ=255) cTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCa < 1:4167159/71‑1 (MQ=255) cTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCa < 1:2091880/71‑1 (MQ=255) tGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:3333398/1‑43 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGaa < 1:4174351/71‑1 (MQ=255) | TGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA > W3110S.gb/155395‑155530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |