| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 236,107 | A→C | 57.1% | E14A (GAA→GCA) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 236,107 | 0 | A | C | 57.1% | 290.3 / 243.9 | 238 | E14A (GAA→GCA) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (42/60); new base C (67/68); total (110/128) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
Only 100 of 248 total aligned reads displayed.
AAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGA > W3110S.gb/236038‑236176 | caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:1523937‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:796969‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:1392250‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGAAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:2724835‑M2/6‑71 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAaccac < 1:2629142/70‑1 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc < 1:169521/70‑1 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:5026272/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:1874823/1‑71 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCg > 1:2717257/1‑70 (MQ=255) gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGt < 1:4334521/71‑1 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca > 1:4621943/1‑63 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:679043/70‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGaa > 1:2329653/1‑71 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1321565/1‑70 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAcc < 1:4417343/71‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:146267/71‑1 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:1148307/1‑68 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:4201602/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:303510/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1370566/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:4782320/1‑67 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:1193491/71‑1 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:4723594/71‑1 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCAAGATGGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:1640650/70‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTgg < 1:3323001/71‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTgg > 1:2240337/1‑71 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTg < 1:930720/70‑1 (MQ=255) ccGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc < 1:3986008/62‑1 (MQ=255) ccGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTg < 1:1789977/69‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg < 1:9448/71‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTa > 1:4806080/1‑54 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTgcg < 1:4462922/68‑1 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGaa > 1:3870250/1‑54 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:4887456/56‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:4792187/1‑71 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:4537857/1‑71 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTa < 1:1730603/47‑1 (MQ=39) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:609177/71‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:5054781/70‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:2569454/1‑69 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc < 1:3456302/40‑1 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACAGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:1055682/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAacc > 1:1021625/1‑35 (MQ=38) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTgg > 1:3705197/1‑55 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACC‑‑AACCACCGGTATGAACCAGATTgg > 1:4561383/1‑53 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:2772506/1‑51 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:834832/1‑71 (MQ=255) aTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa > 1:425740/1‑49 (MQ=39) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:102354/46‑1 (MQ=39) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:3796282/46‑1 (MQ=39) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg < 1:2012014/66‑1 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:65453/50‑1 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:4153577/71‑1 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa > 1:4070628/1‑46 (MQ=39) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTa > 1:4007851/1‑37 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:741927/1‑42 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCa > 1:2992986/1‑44 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:1878242/1‑69 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:3076654/47‑1 (MQ=39) cGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:3206008/71‑1 (MQ=255) cGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:2201257/71‑1 (MQ=255) gCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt > 1:1439995/1‑70 (MQ=255) cAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa > 1:906828/1‑70 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc < 1:4164414/48‑1 (MQ=39) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:3536877/71‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt < 1:5158065/38‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt < 1:2938474/38‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt < 1:3067525/38‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:4718561/52‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt < 1:1091642/69‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt < 1:3632107/69‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:3160487/71‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgag < 1:3891601/71‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:2504193/49‑1 (MQ=39) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2429372/1‑67 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2386687/1‑67 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2618792/1‑67 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:290853/1‑35 (MQ=255) gTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:3294170/71‑1 (MQ=255) gTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:2294303/71‑1 (MQ=255) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATc < 1:5203656/62‑1 (MQ=255) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATc < 1:1548212/62‑1 (MQ=255) tctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATc < 1:586618/61‑1 (MQ=255) tctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt < 1:4592204/70‑1 (MQ=255) tcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa > 1:3397729/1‑47 (MQ=39) tcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg < 1:4240471/56‑1 (MQ=255) cgctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTg > 1:2119570/4‑71 (MQ=255) gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTg < 1:1683837/68‑1 (MQ=255) gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGc < 1:1253689/69‑1 (MQ=255) gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGc < 1:195989/69‑1 (MQ=255) gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGc < 1:4381804/69‑1 (MQ=255) ctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGcc < 1:4761400/70‑1 (MQ=255) tACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt > 1:4831307/1‑63 (MQ=255) aCCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGt > 1:161504/1‑71 (MQ=255) aCCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGt > 1:2066628/1‑71 (MQ=255) ccGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:2445605/1‑42 (MQ=255) ccGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg > 1:4307771/1‑64 (MQ=255) gAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGa < 1:2820690/71‑1 (MQ=255) aaaCCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTg > 1:356658/1‑69 (MQ=255) aaaCCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTg > 1:1115912/1‑69 (MQ=255) | AAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGA > W3110S.gb/236038‑236176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |