Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6310CT100.0% 232.6 / NA 79Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/55);  total (24/55)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATC  >  W3110S.gb/1303566‑1303696
                                                                 |                                                                 
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:4134204/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:3085707/71‑1 (MQ=255)
 aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                             >  1:4197798/1‑71 (MQ=255)
    cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt                                                          <  1:3751967/71‑1 (MQ=255)
     gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                         <  1:1382207/71‑1 (MQ=255)
     gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                         <  1:1388234/71‑1 (MQ=255)
      aacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                        >  1:2985798/1‑71 (MQ=255)
      aacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                        >  1:3159382/1‑71 (MQ=255)
        cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:2707047/63‑1 (MQ=255)
        cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:2891678/63‑1 (MQ=255)
        cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                      <  1:3112995/71‑1 (MQ=255)
         gaaTCGTTCTTGAGCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTg                                                     >  1:2126354/1‑71 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:4398245/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:4751092/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:3642349/71‑1 (MQ=255)
            tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt                                                  <  1:4077883/71‑1 (MQ=255)
              gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                                <  1:1799102/71‑1 (MQ=255)
               ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                               <  1:3212729/71‑1 (MQ=255)
               ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                               <  1:2827406/71‑1 (MQ=255)
                tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTg                                              >  1:3144603/1‑71 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                        <  1:390568/60‑1 (MQ=255)
                  ttGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                 >  1:4056451/1‑66 (MQ=255)
                    gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                             <  1:1746741/68‑1 (MQ=255)
                    gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                             <  1:3699115/68‑1 (MQ=255)
                    gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                           <  1:5204244/70‑1 (MQ=255)
                    gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                           <  1:437070/70‑1 (MQ=255)
                    gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                           <  1:4551709/70‑1 (MQ=255)
                     aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                          <  1:3835502/70‑1 (MQ=255)
                     aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                         <  1:4815427/71‑1 (MQ=255)
                     aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                         <  1:2254944/71‑1 (MQ=255)
                     aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                         <  1:300479/71‑1 (MQ=255)
                      aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                        <  1:2768343/71‑1 (MQ=255)
                      aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                        <  1:1648584/71‑1 (MQ=255)
                      aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                        <  1:4724745/71‑1 (MQ=255)
                       cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                       <  1:2940685/71‑1 (MQ=255)
                       cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                       <  1:253918/71‑1 (MQ=255)
                        gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                       >  1:159093/1‑54 (MQ=255)
                        gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                       >  1:3968392/1‑54 (MQ=255)
                           gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                                    >  1:867791/1‑70 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                >  1:4623229/1‑71 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:836120/71‑1 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                >  1:1996783/1‑71 (MQ=255)
                               gACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                >  1:4340205/1‑70 (MQ=255)
                                aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                  <  1:2175136/67‑1 (MQ=255)
                                 ccTACTTGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                             <  1:1327750/71‑1 (MQ=255)
                                  cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTg                                                     <  1:2378026/46‑1 (MQ=39)
                                  cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                       <  1:1204836/60‑1 (MQ=255)
                                   tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           >  1:3870538/1‑71 (MQ=255)
                                    aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           >  1:4735635/1‑70 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt                                                          <  1:1232800/38‑1 (MQ=37)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                 >  1:787967/1‑47 (MQ=39)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           >  1:4246985/1‑69 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          <  1:193553/70‑1 (MQ=255)
                                      tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                         <  1:2552634/38‑1 (MQ=37)
                                      tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:2998469/44‑1 (MQ=38)
                                      tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt                                                  <  1:2310236/45‑1 (MQ=38)
                                        gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:5059113/61‑1 (MQ=255)
                                        gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                         >  1:4858833/1‑68 (MQ=255)
                                        gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                         >  1:2338575/1‑68 (MQ=255)
                                         aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCg                     >  1:3754932/1‑71 (MQ=255)
                                         aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCg                     >  1:1648434/1‑71 (MQ=255)
                                         aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCg                     <  1:1031558/71‑1 (MQ=255)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                  >  1:3030502/1‑54 (MQ=255)
                                              cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                      >  1:2818252/1‑49 (MQ=39)
                                              cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           <  1:3618903/60‑1 (MQ=255)
                                               gcgAAAACCGTTATTATCTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg               <  1:849765/71‑1 (MQ=255)
                                                cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt              <  1:463619/71‑1 (MQ=255)
                                                     aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtgg         <  1:4530738/71‑1 (MQ=255)
                                                       cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                       <  1:2349060/55‑1 (MQ=255)
                                                        gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                             <  1:2221412/48‑1 (MQ=39)
                                                        gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                             <  1:1218606/48‑1 (MQ=39)
                                                        gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                             <  1:5130972/48‑1 (MQ=39)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          <  1:3116980/48‑1 (MQ=39)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          <  1:2137578/48‑1 (MQ=39)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt   >  1:4666458/1‑71 (MQ=255)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt   >  1:1598445/1‑71 (MQ=255)
                                                            ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc  <  1:3751125/71‑1 (MQ=255)
                                                            ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc  <  1:1187064/71‑1 (MQ=255)
                                                            ttaACTATGTAACCTATTTGCCTATTACTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc  <  1:780365/71‑1 (MQ=255)
                                                                 |                                                                 
TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATC  >  W3110S.gb/1303566‑1303696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: