| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,866,110 | T→A | 100% | intergenic (‑20/+160) | rpoS ← / ← nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,866,110 | 0 | T | A | 100.0% | 181.5 / NA | 61 | intergenic (‑20/+160) | rpoS/nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (23/38); total (23/38) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCT > W3110S.gb/2866044‑2866169 | aaCACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:1093356/71‑1 (MQ=255) cacacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:2377693/1‑71 (MQ=255) cacacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:2361674/1‑71 (MQ=255) cacacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTct > 1:1786065/1‑70 (MQ=255) cacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:4753443/71‑1 (MQ=255) cacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:4857934/71‑1 (MQ=255) acGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctacta < 1:3677922/71‑1 (MQ=255) acGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctacta < 1:3069552/71‑1 (MQ=255) gCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGGCAAATCGTTATCACTGGGTTCCAATTc > 1:4882749/1‑64 (MQ=255) tgtgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCt < 1:3928749/71‑1 (MQ=255) gtgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCt > 1:114964/1‑70 (MQ=255) tgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac > 1:2468860/1‑61 (MQ=255) tgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac > 1:2724185/1‑61 (MQ=255) tgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCttt < 1:3364084/71‑1 (MQ=255) tgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt < 1:5152937/70‑1 (MQ=255) tgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt < 1:296076/70‑1 (MQ=255) tgGCTCCCTGCGATAACAGTTCATCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt < 1:4228079/70‑1 (MQ=255) cTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCg > 1:2203804/1‑71 (MQ=255) cTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCg > 1:2995499/1‑71 (MQ=255) tGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt < 1:4901025/62‑1 (MQ=255) tGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa > 1:1690737/1‑70 (MQ=255) cGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaa < 1:3618779/70‑1 (MQ=255) cGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaa < 1:725070/70‑1 (MQ=255) tAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:407099/49‑1 (MQ=255) tAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:5007232/49‑1 (MQ=255) tAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAAcc > 1:2553854/1‑70 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:798699/1‑45 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGt > 1:893687/1‑60 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGt > 1:4928483/1‑60 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:1565391/70‑1 (MQ=255) aGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTc > 1:4507395/1‑59 (MQ=255) gTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:1944141/40‑1 (MQ=255) ttCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac < 1:4671416/42‑1 (MQ=255) ttCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCaa < 1:3696822/68‑1 (MQ=255) tCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:3979054/1‑40 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAAt < 1:3978063/35‑1 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAAt < 1:4286152/35‑1 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGc < 1:4564678/46‑1 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt > 1:3470666/1‑49 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg < 1:4789111/70‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg < 1:1085035/69‑1 (MQ=255) ctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtc > 1:1270736/1‑71 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCt > 1:1240654/1‑44 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa < 1:1258699/53‑1 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGtt < 1:761103/69‑1 (MQ=255) tCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct > 1:3598037/1‑70 (MQ=255) ccAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCa < 1:3592898/47‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt < 1:2534376/38‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:4516096/47‑1 (MQ=255) aaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct < 1:2732785/63‑1 (MQ=255) aTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAtt < 1:4622267/71‑1 (MQ=255) aTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAtt < 1:2154072/71‑1 (MQ=255) aTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAtt < 1:5172091/71‑1 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAtt < 1:645207/68‑1 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg > 1:3527400/1‑71 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGc < 1:4361072/71‑1 (MQ=255) tCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:1352870/38‑1 (MQ=255) cACTGGGTTCCAATTCTACTTAGGCCTTTTCGTCAAAAACATCAACTCCGTTCTCATCAAATTCCGCATCt > 1:221207/1‑71 (MQ=255) aCTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaaa > 1:1102528/1‑38 (MQ=255) gggTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:2982835/55‑1 (MQ=255) gggTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:38743/55‑1 (MQ=255) | AACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCT > W3110S.gb/2866044‑2866169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |