Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	100%	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	169.4 / NA	58	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (31/27);  total (31/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA  >  W3110S.gb/4563522‑4563656                                                                    |                                                                    tgTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                  <  1:154319/70‑1 (MQ=255) ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                  <  1:430884/71‑1 (MQ=255)    cGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                               >  1:2699073/1‑71 (MQ=255)      gcagcaGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTg                                                             >  1:3517532/1‑71 (MQ=255)          cagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGc                                                         >  1:3030493/1‑71 (MQ=255)            gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc                                                       <  1:3632234/71‑1 (MQ=255)             cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                      >  1:207069/1‑71 (MQ=255)                gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                    <  1:145110/70‑1 (MQ=255)                gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                    >  1:2624245/1‑70 (MQ=255)                gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                    <  1:3066923/70‑1 (MQ=255)                    aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                        <  1:4402721/62‑1 (MQ=255)                    aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                 <  1:562490/69‑1 (MQ=255)                    aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                 <  1:3115212/69‑1 (MQ=255)                       ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCTATTTTACTGTCGCCCATGTGGAacac                                            <  1:4580904/71‑1 (MQ=255)                       ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                                  <  1:4404042/49‑1 (MQ=255)                       ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac                                            <  1:945926/71‑1 (MQ=255)                        gCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                           <  1:3633641/71‑1 (MQ=255)                        gCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg                                           >  1:2961780/1‑71 (MQ=255)                              cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg                                                          >  1:4123070/1‑50 (MQ=255)                              cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg                                                          >  1:3875312/1‑50 (MQ=255)                              cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:306051/1‑63 (MQ=255)                              cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:2156251/1‑63 (MQ=255)                              cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             >  1:2680938/1‑63 (MQ=255)                               gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGt                                                            >  1:3236306/1‑47 (MQ=255)                               gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc                                    >  1:2398974/1‑71 (MQ=255)                                cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                   <  1:5031313/71‑1 (MQ=255)                                   ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt                                                   >  1:4152829/1‑52 (MQ=255)                                   ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGAc                                      <  1:4133977/65‑1 (MQ=255)                                    tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                       >  1:1946368/1‑63 (MQ=255)                                     ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                        >  1:3514647/1‑45 (MQ=255)                                     ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                              >  1:4237505/1‑71 (MQ=255)                                       ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                             <  1:1097557/70‑1 (MQ=255)                                       ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                             <  1:573533/70‑1 (MQ=255)                                       ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                             <  1:1266680/70‑1 (MQ=255)                                         agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTg                                        >  1:1210982/1‑57 (MQ=255)                                         agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAcc                          >  1:1370927/1‑71 (MQ=255)                                         agcagGTCATAAATAGGCTGTAACGCCATTCTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAcc                          >  1:4334806/1‑71 (MQ=255)                                            agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                       <  1:5042374/71‑1 (MQ=255)                                             ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                      <  1:1387413/39‑1 (MQ=255)                                              gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                         <  1:2340970/51‑1 (MQ=255)                                              gTCATAAATAGGCTGTAACGCCATTTTACTCTCGCCCATGTGGAACACGGt                                         <  1:4011457/51‑1 (MQ=255)                                                cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt                                                   <  1:1682570/39‑1 (MQ=255)                                                cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                    >  1:1625908/1‑70 (MQ=255)                                                cATAAATAGGCTGTAACGCCATTTTACTGTCCCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                    >  1:2452200/1‑70 (MQ=255)                                                 aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                               <  1:4534403/42‑1 (MQ=255)                                                   aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:701390/1‑71 (MQ=255)                                                   aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                >  1:1947642/1‑71 (MQ=255)                                                    aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc               >  1:1109093/1‑71 (MQ=255)                                                    aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc               >  1:2704648/1‑71 (MQ=255)                                                     aTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                             <  1:2123373/40‑1 (MQ=255)                                                     aTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg                                >  1:258011/1‑53 (MQ=255)                                                          cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCaa         <  1:4158865/71‑1 (MQ=255)                                                          cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCaa         <  1:3439452/71‑1 (MQ=255)                                                          cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCaa         <  1:863818/71‑1 (MQ=255)                                                           tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCaa         >  1:403441/1‑70 (MQ=255)                                                             tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc      <  1:3953239/71‑1 (MQ=255)                                                               aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                                 >  1:3556288/1‑42 (MQ=255)                                                               aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg    <  1:1652313/71‑1 (MQ=255)                                                                cGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAcc              <  1:438694/60‑1 (MQ=255)                                                                 gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCaa         >  1:2709802/1‑64 (MQ=255)                                                                 gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa  >  1:3158576/1‑71 (MQ=255)                                                                    |                                                                    GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA  >  W3110S.gb/4563522‑4563656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑