Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,461,559 Δ1 bp 7.6% intergenic (‑467/+214) yfcV ← / ← sixA predicted fimbrial‑like adhesin protein/phosphohistidine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,461,5590T. 7.6% 500.3 / 38.4 171intergenic (‑467/+214)yfcV/sixApredicted fimbrial‑like adhesin protein/phosphohistidine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (75/83);  new base . (10/3);  total (85/86)
Fisher's exact test for biased strand distribution p-value = 4.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 173 total aligned reads displayed.

TAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGA  >  W3110S.gb/2461491‑2461629
                                                                     |                                                                      
tAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGa                                                                      >  1:1335253/1‑71 (MQ=255)
     aaGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  <  1:2801843/70‑1 (MQ=255)
     aaGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  <  1:2567751/70‑1 (MQ=255)
     aaGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  <  1:799370/70‑1 (MQ=255)
         ttCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAgtg                                                             >  1:5307921/1‑71 (MQ=255)
         ttCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAgtg                                                             >  1:3036264/1‑71 (MQ=255)
            aaTTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCa                                                          <  1:4722989/71‑1 (MQ=255)
              ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  >  1:1424444/1‑61 (MQ=255)
              ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  >  1:693737/1‑61 (MQ=255)
              ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  >  1:3247929/1‑61 (MQ=255)
              ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  >  1:3149517/1‑61 (MQ=255)
              ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  >  1:1719436/1‑61 (MQ=255)
              ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                                        <  1:4311519/71‑1 (MQ=255)
               tACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                                        <  1:3380604/70‑1 (MQ=255)
                     ctgcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  <  1:2377616/54‑1 (MQ=255)
                       gcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACatta                                               >  1:908650/1‑71 (MQ=255)
                       gcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACatta                                               >  1:4494524/1‑71 (MQ=255)
                       gcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACatta                                               >  1:658953/1‑71 (MQ=255)
                            attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGAt                                                                     <  1:4479747/44‑1 (MQ=255)
                            attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGAt                                                                     <  1:5375587/44‑1 (MQ=255)
                            attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGa                                                    >  1:2213932/1‑61 (MQ=255)
                            attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCa                                           <  1:2693216/70‑1 (MQ=255)
                            attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCAt                                          >  1:765758/1‑71 (MQ=255)
                             ttattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  <  1:3412005/46‑1 (MQ=255)
                             ttattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  <  1:325440/46‑1 (MQ=255)
                             ttattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                                  <  1:4235927/46‑1 (MQ=255)
                                ttaaaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTc                                       <  1:182247/67‑1 (MQ=255)
                                ttCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTc                                       <  1:4979840/70‑1 (MQ=255)
                                ttCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTc                                       <  1:488794/70‑1 (MQ=255)
                                   aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:5165177/1‑43 (MQ=255)
                                   aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:3997308/1‑43 (MQ=255)
                                   aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:5310601/1‑43 (MQ=255)
                                   aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:4253619/1‑43 (MQ=255)
                                   aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:427244/1‑43 (MQ=255)
                                   aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACa                                   >  1:2753339/1‑71 (MQ=255)
                                   aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACa                                   >  1:1223070/1‑71 (MQ=255)
                                   aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACa                                   >  1:570999/1‑71 (MQ=255)
                                      tctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCaa                                     <  1:1049573/66‑1 (MQ=255)
                                      tctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCaa                                     <  1:944629/66‑1 (MQ=255)
                                      tctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCaa                                     <  1:5171574/66‑1 (MQ=255)
                                      tctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCaa                                     <  1:764060/66‑1 (MQ=255)
                                       ctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                >  1:3844746/1‑70 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                                        >  1:4761688/1‑45 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                                        >  1:1493459/1‑45 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:2650901/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:2526458/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:2106066/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:158639/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:4808971/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:4826089/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:4859297/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:2515618/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                                <  1:4023133/69‑1 (MQ=255)
                                        tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTaa                              >  1:4541933/1‑71 (MQ=255)
                                          ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:3289739/1‑36 (MQ=255)
                                          ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:3935027/1‑36 (MQ=255)
                                          ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:3629260/1‑36 (MQ=255)
                                          ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:2006158/1‑36 (MQ=255)
                                          ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:2374944/1‑36 (MQ=255)
                                          ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:5051377/1‑36 (MQ=255)
                                          ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                               >  1:5492885/1‑36 (MQ=255)
                                          ttttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTaa                              <  1:1220853/69‑1 (MQ=255)
                                            ttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt                          >  1:5470755/1‑71 (MQ=255)
                                            ttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt                          >  1:790459/1‑71 (MQ=255)
                                            ttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt                          >  1:2995844/1‑71 (MQ=255)
                                             tctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt                          <  1:988045/70‑1 (MQ=255)
                                             tctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt                          <  1:2320443/70‑1 (MQ=255)
                                               ctcATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCAtt                                         <  1:105042/51‑1 (MQ=9)
                                               ctcATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCAtt                                         <  1:5597410/51‑1 (MQ=9)
                                                 cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                                       >  1:1095640/1‑53 (MQ=17)
                                                 cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                                       >  1:859672/1‑53 (MQ=17)
                                                 cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                                       >  1:2488584/1‑53 (MQ=17)
                                                 cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                                       >  1:3491164/1‑53 (MQ=17)
                                                   ttATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACt                            <  1:5203750/62‑1 (MQ=255)
                                                   ttATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCaa                    >  1:4899016/1‑70 (MQ=255)
                                                     aTCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACtt                           >  1:4267791/1‑61 (MQ=255)
                                                     aTCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAAt                 >  1:4659227/1‑71 (MQ=255)
                                                     aTCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAAt                 >  1:4060613/1‑71 (MQ=255)
                                                           tATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGa            <  1:1187555/71‑1 (MQ=255)
                                                           tATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGa            <  1:4172908/71‑1 (MQ=255)
                                                           tATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGa            <  1:2407439/71‑1 (MQ=255)
                                                            aTTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGa            <  1:3372096/70‑1 (MQ=255)
                                                            aTTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGa            <  1:4788494/70‑1 (MQ=255)
                                                             ttAATAAATGATAATGAGTGTCAAACAGACATTATTc                                            <  1:285269/37‑1 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGa            >  1:5315743/1‑67 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATaag        >  1:3539043/1‑69 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAcg        >  1:3259818/1‑71 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAcg        >  1:1946461/1‑71 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAcg        >  1:2127594/1‑71 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAcg        >  1:4824322/1‑71 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAcg        >  1:3956083/1‑71 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAc         >  1:153946/1‑70 (MQ=255)
                                                               aataaATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGAAAcg        >  1:1444083/1‑71 (MQ=255)
                                                               aataaATGATAATGAGTGTAAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAc         >  1:5458850/1‑70 (MQ=255)
                                                                   aaTGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATAcgcg      >  1:468958/1‑69 (MQ=255)
                                                                    aTGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGAt           <  1:1307386/63‑1 (MQ=255)
                                                                    aTGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGAt           <  1:2573605/63‑1 (MQ=255)
                                                                    aTGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGAt           <  1:3048199/63‑1 (MQ=255)
                                                                    aTGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCag   <  1:2254286/71‑1 (MQ=255)
                                                                     tGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCaga  <  1:2452739/71‑1 (MQ=255)
                                                                     |                                                                      
TAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAATCAAGATACGCGCAGA  >  W3110S.gb/2461491‑2461629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: