Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 1,301,041 Δ1,336 bp 100% insCinsD insC, insD

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 1301041–1302369 1302376 8–1336 72 [0] [0] 71 insC–insD insC,insD

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 13010400 (0.000)31 (0.620) 15/86 NT 100% intergenic (+218/‑90) ychE/insC predicted inner membrane protein/IS2 element protein
?W3110S.gb 1302377 = 0 (0.000)intergenic (+19/‑519) insD/oppA IS2 element protein/oligopeptide transporter subunit

CTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1300964‑1301040
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatattaTTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  W3110S.gb/1302377‑1302446
                                                                                                                                                   
CTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATG                                                                              <  1:3800756/71‑1
CTGCTTCTCATCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATG                                                                              <  1:5471413/71‑1
   CTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAATAATAATTGAAATGATA                                                                           >  1:2474652/1‑71
   CTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATA                                                                           >  1:981242/1‑71
       TCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTAT                                                                       >  1:3780417/1‑71
       TCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTAT                                                                       >  1:2445008/1‑71
       TCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTAT                                                                       >  1:3378320/1‑71
       TCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTAT                                                                       >  1:2034753/1‑71
       TCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTA                                                                        >  1:2663974/1‑70
             CCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTC                                                                 <  1:1642963/71‑1
             CCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTC                                                                 <  1:5244301/71‑1
                TCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCAC                                                              >  1:3976468/1‑71
                        TTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTAT                                                                       <  1:1591176/54‑1
                           TCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGT                                                    <  1:2453276/70‑1
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:1646646/71‑1
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:3192612/71‑1
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:3712803/1‑41
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:3705807/1‑41
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:344656/1‑41
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:4193691/1‑41
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:2757656/1‑41
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:5571135/1‑41
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:1168806/1‑41
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:2111127/1‑41
                                ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            >  1:2284603/1‑41
                                    CATAATCCTGGGCTTGCTGAAGAATAATTGAAATGA                                                                             >  1:4632777/1‑36
                                     ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGT                                          <  1:1330623/70‑1
                                     ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGT                                          <  1:4192373/70‑1
                                     ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGT                                          <  1:212279/70‑1
                                     ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAA                                            >  1:465754/1‑68
                                     ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAA                                            >  1:3551800/1‑68
                                     ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAA                                            >  1:3277588/1‑68
                                     ATAATACTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGT                                          <  1:2704991/70‑1
                                        ATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:2607041/1‑56
                                        ATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:4131012/1‑56
                                        ATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:4984533/1‑56
                                        ATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:5083597/1‑56
                                        ATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:579453/1‑56
                                        ATCCTGGCCTTGCTGAAGAATAATTGAAAAGATATTATTAATTCCACTGCCTTTGG                                                     >  1:4885311/1‑56
                                           CTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:4892201/1‑70
                                           CTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCAC                                                              <  1:4351792/44‑1
                                               GCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGT                                          <  1:2711508/60‑1
                                               GCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTA                                                   >  1:3444860/1‑51
                                                   GCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATC                            <  1:5020869/70‑1
                                                    CTGAAGAATTATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAA                          <  1:538443/71‑1
                                                    CTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAA                          <  1:4149879/71‑1
                                                    CTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGT                                                    <  1:2837196/45‑1
                                                       AAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTG                       >  1:5065149/1‑71
                                                              ATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATT                <  1:4423966/71‑1
                                                              ATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAAT                 <  1:4012964/70‑1
                                                              ATTGAAATGATATTATTAATTCCACTGCCTTTCGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAAT                 <  1:3490059/70‑1
                                                                   AATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGT           <  1:3082404/71‑1
                                                                   AATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGT           <  1:1925601/71‑1
                                                                       ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:4706526/40‑1
                                                                       ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:5498293/40‑1
                                                                       ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:1257536/40‑1
                                                                       ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:2610059/40‑1
                                                                       ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:121135/40‑1
                                                                       ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:3953987/40‑1
                                                                       ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:3802418/40‑1
                                                                       ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:3698898/40‑1
                                                                        TATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      <  1:936007/39‑1
                                                                         ATTATTAATTCCACTGGCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTG          >  1:4320938/1‑66
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTG          >  1:3089571/1‑66
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTG          >  1:5435211/1‑66
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTG          >  1:380586/1‑66
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:108023/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:2382669/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:3200691/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:3241697/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:3448428/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:4846805/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:4801210/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:4652054/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                    >  1:4302627/1‑40
                                                                         ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGCGCTAAA                                    >  1:443381/1‑40
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCgc  >  1:4614251/1‑69
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:1034065/1‑71
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:1489391/1‑71
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:4807906/1‑71
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:1474340/1‑71
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:5502985/1‑71
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:2473631/1‑71
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:2581859/1‑71
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:615550/1‑71
                                                                            ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTG            >  1:5458138/1‑61
                                                                                                                                                   
CTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1300964‑1301040
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatattaTTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  W3110S.gb/1302377‑1302446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.