| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,899,219 | T→C | 6.4% | intergenic (‑290/‑29) | ygcW ← / → yqcE | predicted deoxygluconate dehydrogenase/predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,899,219 | 0 | T | C | 6.4% | 273.3 / 7.8 | 95 | intergenic (‑290/‑29) | ygcW/yqcE | predicted deoxygluconate dehydrogenase/predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (38/50); new base C (4/2); total (42/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGTTGGATAACCCTCGCGAT > W3110S.gb/2899152‑2899288 | tattatTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttttt > 1:1089208/1‑71 (MQ=255)tattatTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttttt > 1:2737241/1‑71 (MQ=255) tCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTAt > 1:3467548/1‑71 (MQ=255) tCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTAt > 1:1160193/1‑71 (MQ=255) cAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTAt > 1:5292529/1‑70 (MQ=255) ttACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:4871587/71‑1 (MQ=255) ttACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:2568492/71‑1 (MQ=255) tACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa > 1:2462221/1‑70 (MQ=255) aCTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACaaa > 1:2710514/1‑71 (MQ=255) aCTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACaa < 1:1160110/70‑1 (MQ=255) cTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACaa < 1:2603393/69‑1 (MQ=255) cTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAAt < 1:1398980/71‑1 (MQ=255) tAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:3792312/67‑1 (MQ=255) gagaCCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATggg < 1:1452247/71‑1 (MQ=255) gagaCCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATggg < 1:5052098/71‑1 (MQ=255) gagaCCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATggg < 1:3463715/71‑1 (MQ=255) gagaCCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATgg < 1:2814415/70‑1 (MQ=255) gagaCCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATgg > 1:3391526/1‑70 (MQ=255) agaCCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATggg < 1:3734170/70‑1 (MQ=255) ccTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTCTTTTGTCTATTACaaa > 1:3252717/1‑63 (MQ=255) ccTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTCTTTTGTCTATTACAAATGGGAAt > 1:113416/1‑70 (MQ=255) ccTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTCTTTTGTCTATTACAAATGGGAATa > 1:2440464/1‑71 (MQ=255) ccTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTCTTTTGTCTATTACAAATGGGAATa > 1:5169038/1‑71 (MQ=255) agaAAAGTAATCTACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACg < 1:2183938/69‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTAc < 1:816450/56‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTAc < 1:4877781/56‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAAt < 1:1159075/60‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAAt < 1:3452414/60‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAAt < 1:1746814/60‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAAt < 1:457458/60‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAAt < 1:3127687/60‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACg < 1:246130/69‑1 (MQ=255) agaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGa > 1:1176007/1‑70 (MQ=255) gaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTAc < 1:291594/55‑1 (MQ=255) gaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACatat < 1:2830164/59‑1 (MQ=255) gaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACg < 1:5144569/68‑1 (MQ=255) gaAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGa < 1:5206209/69‑1 (MQ=255) aaaGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAAt < 1:786630/57‑1 (MQ=255) aTCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAacac < 1:4891063/71‑1 (MQ=255) aTCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAaca > 1:2786988/1‑70 (MQ=255) tCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:2975447/47‑1 (MQ=255) tCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:1438933/47‑1 (MQ=255) tCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:1391319/47‑1 (MQ=255) cACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAAt < 1:971820/48‑1 (MQ=255) aCCTGAAAGGGCGGCTAACTTTTCTCTTTTTTTTGTCTATTACatat < 1:5416093/47‑1 (MQ=39) ccTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATAc < 1:5333080/54‑1 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTa > 1:4967256/1‑37 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTa > 1:2218778/1‑37 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTa > 1:4973720/1‑37 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTa > 1:4081823/1‑37 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTa > 1:4076247/1‑37 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:961981/42‑1 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:787147/42‑1 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACa < 1:3768515/42‑1 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATAc < 1:1968504/53‑1 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCaa < 1:2629526/62‑1 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCaa < 1:2559192/62‑1 (MQ=255) cTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCaa < 1:5128491/62‑1 (MQ=255) gAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:4052902/1‑55 (MQ=255) gAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:3696312/1‑55 (MQ=255) gAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:3702919/1‑55 (MQ=255) gAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:147898/1‑55 (MQ=255) gAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:4353309/1‑55 (MQ=255) gAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:501379/1‑55 (MQ=255) aaaGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGc < 1:877223/57‑1 (MQ=255) aaaGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGc < 1:3336415/57‑1 (MQ=255) gggCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGa < 1:5329822/49‑1 (MQ=255) gggCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGa < 1:269412/49‑1 (MQ=255) gggCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGa < 1:4944490/49‑1 (MQ=255) ggcggcTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGcc > 1:2873046/1‑71 (MQ=255) gcggcTTACTTTTCTCTTTCTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCg < 1:4862263/71‑1 (MQ=255) gcggcTTACTTTTCTCTTTCTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCg < 1:4918580/71‑1 (MQ=255) gcTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGTTg < 1:2856981/71‑1 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:5431655/1‑44 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:5461619/1‑44 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:556038/1‑44 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:1047768/1‑44 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:4898655/1‑44 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:4253855/1‑44 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAaca > 1:5086207/1‑51 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGt > 1:3270625/1‑67 (MQ=255) ttACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGTTGGa > 1:73121/1‑71 (MQ=255) aCTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGTTGGAt < 1:3315284/70‑1 (MQ=255) aCTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGTTGGAt > 1:349325/1‑70 (MQ=255) tttCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCaa > 1:957849/1‑44 (MQ=255) ttCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:4287013/1‑38 (MQ=255) ttCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:3538330/1‑38 (MQ=255) ttCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:3730951/1‑38 (MQ=255) ttCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:332106/1‑38 (MQ=255) ttCTCTTTTTTTTGTCTATTACAAATGGGAATACGata > 1:5281832/1‑38 (MQ=255) tttttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGcc < 1:4205329/53‑1 (MQ=255) tttttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGcc < 1:4704669/53‑1 (MQ=255) ttttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGTTGGATAACCCt > 1:686025/1‑65 (MQ=255) tttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCg > 1:4423776/1‑52 (MQ=255) tttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCg > 1:4913710/1‑52 (MQ=255) tttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGt > 1:3862847/1‑53 (MQ=255) tttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGt > 1:917465/1‑53 (MQ=255) tttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGt > 1:3556639/1‑53 (MQ=255) tttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGt > 1:5007978/1‑53 (MQ=255) tttttGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGTTGGATAACCCTCGCGAt < 1:4391515/70‑1 (MQ=255) | TATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGAATACGATATGCAACACAACTCATATCGCCGTTGGATAACCCTCGCGAT > W3110S.gb/2899152‑2899288 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |